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Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study

Year 2019, Volume: 32 Issue: 3, 792 - 800, 01.09.2019
https://doi.org/10.35378/gujs.467166

Abstract

The importance of secondary metabolites obtained from fungi is increasing day by day in terms of human health. Many physiological and pharmacological effects, mainly antimicrobial, anticancer and antioxidant properties of these compounds have been reported. Aim of this study is to determine the anticancer, antioxidant and antimicrobial effects of aspergillin PZ and terphenyllin compounds, which are isolated from Aspergillus and also subjected to limited number of studies. The antimicrobial activity of aspergillin PZ and terphenyllin compounds was determined by using disc diffusion method using different bacteria. The antioxidant property of the compounds was determined by measuring the level of DPPH free radical scavenging. Cytotoxic activity was determined by experiments on human prostate cancer cell lines (PC3 and LNCaP) and over cancer cell line (A2780). Both compounds showed low antimicrobial activity on test bacteria (approximately 2-3 mm zone). High concentrations of applied compounds showed apparent DPPH free radical scavenging activity, while % scavenging activity was quite low at low concentrations. Both compounds showed significant anticancer activity on cancer cell lines (p <0.05). Our results suggest that these two compounds have important biological properties due to their antioxidant and anticancer activities.

References

  • Brakhage, A.A., "Regulation of fungal secondary metabolism", Nat Rev Microbiol, 11(1): 21-32, (2013).
  • Öztürk, A., Çopur, Ö.U., "Mantar bileşenlerinin terapötik etkileri", Bahçe, 37(2): 11-17, (2008).
  • Keller, N.P., Turner, G., Bennett, J.W., "Fungal secondary metabolism - from biochemistry to genomics", Nat Rev Microbiol, 3(12): 937-47, (2005).
  • Zhang, Y., Wang, T., Pei, Y., Hua, H., Feng, B., "Aspergillin PZ, a novel isoindole-alkaloid from Aspergillus awamori", J Antibiot (Tokyo), 55(8): 693-5, (2002).
  • Akyuz, M., Kirbag, S., "Antimicrobial activity of Pleurotus eryngii var. ferulae grown on various agro-wastes", EurAsian Journal of BioSciences., 3: 58-63, (2009).
  • Brand-Williams, W., Cuvelier, M.E., Berset, C., "Use of a free radical method to evaluate antioxidant activity", LWT - Food Science and Technology, 28(1): 25-30, (1995).
  • Tekin, S., Erden, Y., Sandal, S., Yilmaz, B., "Is irisin an anticarcinogenic peptide?", Medicine Science, 4(2): (2015).
  • Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65(1): 55-63, (1983).
  • Newman, D.J., Cragg, G.M., "Natural Products as Sources of New Drugs from 1981 to 2014", J Nat Prod, 79(3): 629-61, (2016).
  • Bills, G.F., Gloer, J.B., "Biologically Active Secondary Metabolites from the Fungi", Microbiol Spectr, 4(6): (2016).
  • Singh, S.B., Jayasuriya, H., Dewey, R., Polishook, J.D., Dombrowski, A.W., Zink, D.L., Guan, Z., Collado, J., Platas, G., Pelaez, F., Felock, P.J., Hazuda, D.J., "Isolation, structure, and HIV-1-integrase inhibitory activity of structurally diverse fungal metabolites", J Ind Microbiol Biotechnol, 30(12): 721-31, (2003).
  • Yen, G.C., Chang, Y.C., Sheu, F., Chiang, H.C., "Isolation and characterization of antioxidant compounds from Aspergillus candidus broth filtrate", J Agric Food Chem, 49(3): 1426-31, (2001).
  • Yen, G.C., Chiang, H.C., Wu, C.H., Yeh, C.T., "The protective effects of Aspergillus candidus metabolites against hydrogen peroxide-induced oxidative damage to Int 407 cells", Food Chem Toxicol, 41(11): 1561-7, (2003).
  • Duran-Cabral, M., Fernandez-Jalao, I., Estevez-Santiago, R., Olmedilla Alonso, B., "Assessment of individual carotenoid and vitamin A dietary intake in overweight and obese Dominican subjects", Nutrición Hospitalaria, 34(2): 407-415, (2017).
  • Chen, L., Liu, Y.-T., Song, B., Zhang, H.-W., Ding, G., Liu, X.-Z., Gu, Y.-C., Zou, Z.-M., "Stereochemical determination of new cytochalasans from the plant endophytic fungus Trichoderma gamsii", Fitoterapia, 96(Supplement C): 115-122, (2014).
Year 2019, Volume: 32 Issue: 3, 792 - 800, 01.09.2019
https://doi.org/10.35378/gujs.467166

Abstract

References

  • Brakhage, A.A., "Regulation of fungal secondary metabolism", Nat Rev Microbiol, 11(1): 21-32, (2013).
  • Öztürk, A., Çopur, Ö.U., "Mantar bileşenlerinin terapötik etkileri", Bahçe, 37(2): 11-17, (2008).
  • Keller, N.P., Turner, G., Bennett, J.W., "Fungal secondary metabolism - from biochemistry to genomics", Nat Rev Microbiol, 3(12): 937-47, (2005).
  • Zhang, Y., Wang, T., Pei, Y., Hua, H., Feng, B., "Aspergillin PZ, a novel isoindole-alkaloid from Aspergillus awamori", J Antibiot (Tokyo), 55(8): 693-5, (2002).
  • Akyuz, M., Kirbag, S., "Antimicrobial activity of Pleurotus eryngii var. ferulae grown on various agro-wastes", EurAsian Journal of BioSciences., 3: 58-63, (2009).
  • Brand-Williams, W., Cuvelier, M.E., Berset, C., "Use of a free radical method to evaluate antioxidant activity", LWT - Food Science and Technology, 28(1): 25-30, (1995).
  • Tekin, S., Erden, Y., Sandal, S., Yilmaz, B., "Is irisin an anticarcinogenic peptide?", Medicine Science, 4(2): (2015).
  • Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65(1): 55-63, (1983).
  • Newman, D.J., Cragg, G.M., "Natural Products as Sources of New Drugs from 1981 to 2014", J Nat Prod, 79(3): 629-61, (2016).
  • Bills, G.F., Gloer, J.B., "Biologically Active Secondary Metabolites from the Fungi", Microbiol Spectr, 4(6): (2016).
  • Singh, S.B., Jayasuriya, H., Dewey, R., Polishook, J.D., Dombrowski, A.W., Zink, D.L., Guan, Z., Collado, J., Platas, G., Pelaez, F., Felock, P.J., Hazuda, D.J., "Isolation, structure, and HIV-1-integrase inhibitory activity of structurally diverse fungal metabolites", J Ind Microbiol Biotechnol, 30(12): 721-31, (2003).
  • Yen, G.C., Chang, Y.C., Sheu, F., Chiang, H.C., "Isolation and characterization of antioxidant compounds from Aspergillus candidus broth filtrate", J Agric Food Chem, 49(3): 1426-31, (2001).
  • Yen, G.C., Chiang, H.C., Wu, C.H., Yeh, C.T., "The protective effects of Aspergillus candidus metabolites against hydrogen peroxide-induced oxidative damage to Int 407 cells", Food Chem Toxicol, 41(11): 1561-7, (2003).
  • Duran-Cabral, M., Fernandez-Jalao, I., Estevez-Santiago, R., Olmedilla Alonso, B., "Assessment of individual carotenoid and vitamin A dietary intake in overweight and obese Dominican subjects", Nutrición Hospitalaria, 34(2): 407-415, (2017).
  • Chen, L., Liu, Y.-T., Song, B., Zhang, H.-W., Ding, G., Liu, X.-Z., Gu, Y.-C., Zou, Z.-M., "Stereochemical determination of new cytochalasans from the plant endophytic fungus Trichoderma gamsii", Fitoterapia, 96(Supplement C): 115-122, (2014).
There are 15 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Biology
Authors

Yavuz Erden 0000-0002-2807-6096

Suat Tekın 0000-0002-2757-1802

Kevser Betul Ceylan This is me 0000-0002-6006-727X

Cigdem Tekın This is me 0000-0002-2757-1802

Sevda Kırbag 0000-0002-4337-8236

Publication Date September 1, 2019
Published in Issue Year 2019 Volume: 32 Issue: 3

Cite

APA Erden, Y., Tekın, S., Betul Ceylan, K., Tekın, C., et al. (2019). Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study. Gazi University Journal of Science, 32(3), 792-800. https://doi.org/10.35378/gujs.467166
AMA Erden Y, Tekın S, Betul Ceylan K, Tekın C, Kırbag S. Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study. Gazi University Journal of Science. September 2019;32(3):792-800. doi:10.35378/gujs.467166
Chicago Erden, Yavuz, Suat Tekın, Kevser Betul Ceylan, Cigdem Tekın, and Sevda Kırbag. “Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in Vitro Study”. Gazi University Journal of Science 32, no. 3 (September 2019): 792-800. https://doi.org/10.35378/gujs.467166.
EndNote Erden Y, Tekın S, Betul Ceylan K, Tekın C, Kırbag S (September 1, 2019) Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study. Gazi University Journal of Science 32 3 792–800.
IEEE Y. Erden, S. Tekın, K. Betul Ceylan, C. Tekın, and S. Kırbag, “Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study”, Gazi University Journal of Science, vol. 32, no. 3, pp. 792–800, 2019, doi: 10.35378/gujs.467166.
ISNAD Erden, Yavuz et al. “Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in Vitro Study”. Gazi University Journal of Science 32/3 (September 2019), 792-800. https://doi.org/10.35378/gujs.467166.
JAMA Erden Y, Tekın S, Betul Ceylan K, Tekın C, Kırbag S. Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study. Gazi University Journal of Science. 2019;32:792–800.
MLA Erden, Yavuz et al. “Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in Vitro Study”. Gazi University Journal of Science, vol. 32, no. 3, 2019, pp. 792-00, doi:10.35378/gujs.467166.
Vancouver Erden Y, Tekın S, Betul Ceylan K, Tekın C, Kırbag S. Antioxidant, Antimicrobial and Anticancer Activities of the Aspergillin PZ and Terphenyllin Secondary Metabolites: An in vitro Study. Gazi University Journal of Science. 2019;32(3):792-800.