Year 2020, Volume 21 , Issue 2, Pages 301 - 309 2020-09-15

Mycorrhizal fungi diversity of Serapias orientalis
Serapias orientalis’in mikorizal fungus çeşitliliği

Vildan AKIN MUTLU [1] , Hamdiye ŞEHİRLİ [2] , Yasemin ÖZDENER KÖMPE [3]


Orchids are under a serious threat of extinction as a result of both excessive collecting and severe destruction of their habitats. Studies conducted have shown that the protection of orchids depends on the presence of mycorrhizal fungal biodiversity. In this study, molecular identification of mychorrhizal fungi of Serapias orieantalis roots taken from different habitats within the borders of 4 provinces (Samsun, Ordu, Giresun, Trabzon) in the Central and Eastern Black Sea region was conducted. Plant roots were collected in the spring of 2015 and first examined under a microscope and then mycorrhizal fungi were isolated. Purified isolates were developed in PDA (potato dectrose agar) medium in order to determine colony characteristics (colony type, colony color), hif structure (hif diameter and branching structure) and core numbers. Cluster analysis (UPGMA) method was applied to group the isolates according to their morphological characteristics and a cladogram was created. Nuclear ribozomal DNA (rDNA)’nın ITS 1-5.8S-ITS 2 gene region was amplified using ITS 1/ITS 4 primers in order to identify isolates of 3 groups formed according to morphological data at the molecular level. Based on the DNA sequence, 13 Rhizoctonia-like isolates were closely related to the Tulasnella genus, and 2 Rhizoctonia-unlike isolates were closely related to the Fusarium oxysporum.
Orkideler, yumrularının aşırı toplanması ve habitatlarının ciddi şekilde tahrip olması sonucu yok olma tehdidi altındadır. Doğada orkidelerin korunması mikorizal fungus biyolojik çeşitliliğinin varlığına bağlıdır. Bu çalışmada, Orta ve Doğu Karadeniz bölgesinde 4 ilin (Samsun, Ordu, Giresun, Trabzon) sınırları içinde farklı habitatlardan toplanan Serapias orieantalis (Greuter ) Bauman H, Künkele köklerindeki mikorizal fungusların moleküler tanımlaması yapılmıştır. Bitki kökleri 2015 bahar aylarında toplanmış ve önce mikroskopta incelenerek mikorizal funguslar izole edilmiştir. Saflaştırılan izolatlar, koloni özellikleri (koloni tipi, koloni rengi), hif yapısı (hif çapı ve dallanma yapısı) ve çekirdek sayılarının belirlenmesi amacıyla PDA (patates dektroz agar) ortamında geliştirilmiştir. İzolatları morfolojik özelliklerine göre gruplandırmak için küme analizi (UPGMA) yöntemi uygulanmış ve kladogram oluşturulmuştur. Kümeleme analizine göre oluşturulan 3 grubun izolatlarını moleküler düzeyde tanımlayabilmek için ITS-1 ve ITS-4 primerleri kullanılarak nüklear ribozomal DNA (rDNA)’nın ITS1-5.8S-ITS2 gen bölgesi çoğaltılmıştır. DNA dizilimine dayanarak, 13 Rhizoctonia benzeri izolatın Tulasnella cinsi ile 2 Rhizoctonia benzeri olmayan izolatın Fusarium oxysporum Schltdl. türü ile yakın ilişkili olduğu bulunmuştur.
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Primary Language tr
Subjects Biology
Journal Section Research Article
Authors

Orcid: 0000-0002-6330-105X
Author: Vildan AKIN MUTLU (Primary Author)
Institution: T.C. MİLLİ EĞİTİM BAKANLIĞI
Country: Turkey


Orcid: 0000-0003-1053-0817
Author: Hamdiye ŞEHİRLİ
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0003-1649-4298
Author: Yasemin ÖZDENER KÖMPE
Institution: ONDOKUZ MAYIS ÜNİVERSİTESİ
Country: Turkey


Dates

Publication Date : September 15, 2020

Bibtex @research article { artvinofd701282, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, eissn = {2146-698X}, address = {}, publisher = {Artvin Çoruh University}, year = {2020}, volume = {21}, pages = {301 - 309}, doi = {10.17474/artvinofd.701282}, title = {Serapias orientalis’in mikorizal fungus çeşitliliği}, key = {cite}, author = {Akın Mutlu, Vildan and Şehi̇rli̇, Hamdiye and Özdener Kömpe, Yasemin} }
APA Akın Mutlu, V , Şehi̇rli̇, H , Özdener Kömpe, Y . (2020). Serapias orientalis’in mikorizal fungus çeşitliliği . Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi , 21 (2) , 301-309 . DOI: 10.17474/artvinofd.701282
MLA Akın Mutlu, V , Şehi̇rli̇, H , Özdener Kömpe, Y . "Serapias orientalis’in mikorizal fungus çeşitliliği" . Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 21 (2020 ): 301-309 <http://ofd.artvin.edu.tr/en/pub/issue/54132/701282>
Chicago Akın Mutlu, V , Şehi̇rli̇, H , Özdener Kömpe, Y . "Serapias orientalis’in mikorizal fungus çeşitliliği". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 21 (2020 ): 301-309
RIS TY - JOUR T1 - Serapias orientalis’in mikorizal fungus çeşitliliği AU - Vildan Akın Mutlu , Hamdiye Şehi̇rli̇ , Yasemin Özdener Kömpe Y1 - 2020 PY - 2020 N1 - doi: 10.17474/artvinofd.701282 DO - 10.17474/artvinofd.701282 T2 - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JF - Journal JO - JOR SP - 301 EP - 309 VL - 21 IS - 2 SN - 2146-1880-2146-698X M3 - doi: 10.17474/artvinofd.701282 UR - https://doi.org/10.17474/artvinofd.701282 Y2 - 2020 ER -
EndNote %0 Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Serapias orientalis’in mikorizal fungus çeşitliliği %A Vildan Akın Mutlu , Hamdiye Şehi̇rli̇ , Yasemin Özdener Kömpe %T Serapias orientalis’in mikorizal fungus çeşitliliği %D 2020 %J Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi %P 2146-1880-2146-698X %V 21 %N 2 %R doi: 10.17474/artvinofd.701282 %U 10.17474/artvinofd.701282
ISNAD Akın Mutlu, Vildan , Şehi̇rli̇, Hamdiye , Özdener Kömpe, Yasemin . "Serapias orientalis’in mikorizal fungus çeşitliliği". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 21 / 2 (September 2020): 301-309 . https://doi.org/10.17474/artvinofd.701282
AMA Akın Mutlu V , Şehi̇rli̇ H , Özdener Kömpe Y . Serapias orientalis’in mikorizal fungus çeşitliliği. AÇÜOFD. 2020; 21(2): 301-309.
Vancouver Akın Mutlu V , Şehi̇rli̇ H , Özdener Kömpe Y . Serapias orientalis’in mikorizal fungus çeşitliliği. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi. 2020; 21(2): 301-309.