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Türkiye'den Helicoverpa armigera single nükleopolihedrovirüs izolatının izolasyonu, karakterizasyonu ve patojenitesi

Year 2023, Volume: 24 Issue: 1, 187 - 195, 15.05.2023
https://doi.org/10.17474/artvinofd.1270318

Abstract

Pamuk kurdu, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), pamuk, bahçe bitkileri, çeşitli sebze bitkileri ve orman ağaçları gibi tarımsal ürünlerde önemli bir zararlıdır. Polifag bir zararlı olan H. armigera’nın çok sayıda ölü ve canlı larva ve pupa örneği Türkiye’de Adana’dan toplanmıştır. Kadavra derilerinin kırılgan olduğu ve vücut dokularının da sıvılaştığı görüldü. Faz-kontrast mikroskobu ile yapılan çalışmalar, kadavralarda çok yoğun bir bakülovirüs enfeksiyonu ortaya çıkardı. Transmisyon elektron mikroskobu, yeni bakülovirüsün oklüzyon yapılarında, zarf içinde bir nükleokapside sahip olduğunu gösterdi. Bu nedenle izolata Helicoverpa armigera single nükleopolihedrovirüs-D (HearSNPV-D) adı verildi. Polihedrin ve geç ekspresyon faktörü-8 genlerine göre yapılan filogenetik analiz, yeni izolatın HearSNPV Çin ve Türkiye izolatı ile yakın ilişki içinde olduğunu ortaya koydu. Yeni izolat, kendi konakçısı Helicoverpa armigera’nın yanısıra Hyphantria cunea, Malacosoma neustria, Lymantria dispar, Thaumetopea pityocampa ve Spodoptera exigua olmak üzere toplam altı farklı böcek üzerinde test edildi. Biyoanaliz çalışmaları, HearSNPV-D’nin H. armigera’ya karşı öldürücü olduğunu ve dar bir konakçı aralığına sahip olduğunu gösterdi. LC50 değeri H. armigera ve S. exigua için sırasıyla 0.7 × 105 OBs/ml ve 1.9 × 107 OBs/ml olarak belirlendi. HearSNPV-D tarafından konakçı olmayan türlerde anlamlı olmayan ölüm gözlemlendi, bu da HearSNPV-D’nin oldukça spesifik doğasını ortaya çıkarmıştır. Bu nedenle HearSNPV-D, H. armigera için spesifik, çevre dostu bir kontrol ajanı olarak kullanılabilir.

References

  • Abid AD, Saeed S, Zaka SM, Shahzad S, Naqqash MN, Iqbal N (2022) Host range and pathogenicity potential of Helicoverpa armigera nucleopolyhedrovirus (hanpv) to lepidopterous pests of cotton. Journal of King Saud University - Science, 34 (2), Art. No. 101740.
  • Adams JR, Mcclintock JT (1991) Baculoviridae. Nuclear Polyhedrosis Viruses. In: Adams JR, Bonami JR (eds) Atlas of Invertebrate Viruses, Boca Raton, Florida, CRC Press, pp 87-204.
  • Bergomaz B, Boppre´ M (1986) A simple insect diet for rearing Arctiidae and other moths. J Lepid Soc, 40: 131-137.
  • Bilimoria SL (1991) The biology of nuclear polyhedrosis viruses. In: Kurstak E (ed) Viruses of Invertebrates, Marcel Dekker Inc., New York.
  • Black BC, Brennan LA, Dierks LA, Gard IE (1997) Commercialization of baculoviral insecticides. In: Miller LK (ed) The Baculoviruses, Plenum Press, New York.
  • Caballero P, Zuidema D, Santiago-Alvarez C, Vlak JM (1992) Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Sci Technol, 2:145-157. Cherry A, Cock MJW, Van den Berg H, Kfir R (2003) Biological control of Helicoverpa armigera in Africa. In: Neuenschwander P, Borgemeister C, Langewald. J. (eds) United Kingdom, Wallingford. Cooper D, Cory JS, Theilmann DA, Myers JH (2003) Nucleopolyhedroviruses of forest and western tent caterpillars: cross-infectivity and evidence for activation of latent virus in high-density field populations. Ecological Entomology, 28: 41-50.
  • Cory JS, Green BM, Paul RK, Hunter-Fujita F (2005) Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. J Invertebr Pathol, 89: 101-111.
  • Cory JS, Myers JH (2003) The ecology and evolution of insect baculoviruses. Annual Review of Ecology, Evolution, and Systematics, 34:239-272.
  • Cunningham JC (1998) Insect Viruses and Pest Management. In: Hunter-Fujita FR, Entwistle PF, Evans HF, Crook NE (eds) John Wiley & Sons, Inc., New York.
  • Dias PM, Loureiro ESL, Pessoa LGA, Neto FMO, Tosta RAS, Teodoro PE (2019) Interactions between Fungal-Infected Helicoverpa armigera and the Predator Chrysoperla externa. Insects, 10:309.
  • Eroglu GB, Demir I, Demirbag Z (2018) A novel alphabaculovirus isolated from the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae): characterization and pathogenicity. Biologia, 73: 545-551.
  • Eroglu GB, Nalcacioglu R, Demirbag Z (2019) A new Helicoverpa armigera nucleopolyhedrovirus isolate from Heliothis peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) in Turkey. Turk J Biol, 43:340-348.
  • Federici B (1995) Baculovirus pathogenesis. In: Miller LK (ed) The Baculoviruses, Plenum Press.
  • Ferrelli ML, Taibo C, Fichetti P, Sciocco Cap A, Arneodo JD (2016). Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology, 138: 89–93.
  • Fuxa JR, Richter AR, Srother MS (1993) Detection of Anticarsia gemmatalis nuclear polyhedrosis virus in predatory arthropods and parasitoids after viral release in Louisiana soybeen. Journal of Entomological Science, 28:51-60.
  • Gencer D, Bayramoglu Z, Mutlu C, Demir I (2022) Isolation and characterization of novel Spodoptera exigua nucleopolyhedrovirus strains in Turkey and their potential for use in biological control. Acta Virologica, 66(4):360-368.
  • Gencer D, Bayramoglu Z, Nalcacıoglu R, Kleespies RG, Demirbag Z, Demir I (2018) Characterization of three alphabaculovirus isolates from the gypsy moth, Lymantria dispar dispar (Lepidoptera: Erebidae), in Turkey. Biocontrol Science and Technology, 28 (2):107-121.
  • Haase S, Sciocco-Cap A, Romanowski V (2015) Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives. Viruses, 7:2230-2267.
  • Haile F, Nowatzki T, Storer N (2021) Overview of pest status, po-tential risk, and management considerations of Helicoverpa armigera (Lepidoptera: Noctuidae) for U.S. Soybean Production. Journal of Integrated Pest Management, 12.
  • Hall TA (1999) Bioedit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98.
  • Harrison R, Hoover K (2012) Baculoviruses and other occluded insect viruses. In: Vega FE, Kaya HK (eds) Insect Pathology, Academic Press. Hughes PR, Van Beek NAM, Wood HA (1986) A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctuid larvae. Journal of Invertebrate Pathology, 83: 187-192.
  • Il’inykh AV, Ul’yanova EG (2005) Latency of baculoviruses. Biology Bulletin, 32(5): 496-502.
  • Jacob A, Subramanian TR (1972) Nuclear polyhedrosis on some Lepidoptera. Curr Sci, 41:536.
  • Jehle JA, Lange M, Wang H, Hu Z, Wang Hauschild R (2006) Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346:180-193.
  • Kioukia N, Nienow AW, Emery AN, Al-Rubeai M (1995) Physiological and environmental factors affecting the insect cells and infection with baculovirus. Journal of Biotechnology, 38: 243-251.
  • Kumar CS, Ranga Rao GV, Sireesha K, Kumar PL (2011) Isolation and characterization of baculoviruses from three major lepidopteran pests in the semiarid tropics of India. Indian Journal of Virology, 22:29-36.
  • Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874.
  • Luttrell RG, Jackson RE (2012) Helicoverpa zea and Bt cotton in the United States. GM. Crops, 3: 213–227.
  • Mardani-Talaee M, Nouri-Ganbalani G, Naseri B, Hassanpour M, Mottaghinia L, Mansouri S (2016) Comprative life table parameters of the Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) on corn hybrids under laboratory conditions. J Entomol Soc Iran, 35(4):17-27.
  • Martin T, Ochou GO, Djihinto A, Traore D, Togola M, Vassal JM, Vaissayre M, Fournier D (2005) Controlling an insecticide-resistant bollworm in West Africa. Agric Ecosystems Environ, 107(4): 409-411.
  • Milks ML (1997) Comparative biology and susceptibility of cabbage looper (Lepidoptera: Noctuidae) lines to a nuclear polyhedrosis virus. Environ Entomol, 26:839-848.
  • Nakai M, Kunimi Y (1997) Granulosis virus infection of the smaller tea tortrix (Lepidoptera: Tortricidae): Effect on the development of the endoparasitoid, Ascogaster reticulatus (Hymenoptera: Braconidae). Biological Control, 8:74-80.
  • Nawaz A, Ali H, Sufyan M, Gogi MD, Arif MJ, Ranjha MH, Arshid M, Waseem M, Mustafa T, Qasim M, Rizwan M, Zaynab M, Khan KA, Ghramh HA (2019) Comparative bio-efficacy of nuclear polyhedrosis virus (NPV) and Spinosad against American bollwormm, Helicoverpa armigera (Hubner). Revista Brasileira de Entomologia, 63 (4): 277-282.
  • O'Reilly DR, Miller LK, Luckow VA (1992) Baculovirus Expression Vectors. Laboratory Manual, Oxford University Press, New York.
  • Ribeiro HC, Pavan O, Moutri A (1997) Comparative susceptibility of two different hosts to genotypic variants of the Anticarsia gemmatalis nuclear polyhedrosis virus. Entomol Exp Appl, 83: 233-237.
  • Rodríguez VA, Belaich MN, Quintana G, Sciocco-Cap A, Ghiringhelli PD (2012) Isolation and Characterization of a Nucleopolyhedrovirus from Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae), Int J Virol Mol Biol, 1:28-34.
  • Shapiro M, Robertson JL (1991) Natural variability of three geographic isolates of gypsy moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus. J Econ Entomol, 84:71-75.
  • Sharma H (2005) Heliothis/Helicoverpa management: Emerging trends and prospects for future research. Oxford& IBH Publishing Co.
  • Somasekar S, Jayapragasam M, Rabindra RJ (1993) Characterization of five Indian isolates of the nuclear polyhedrosis virus of Helicoverpa armigera. Phytoparasitica, 21:333-337.
  • Sudhakar S, Mathavan S (1999) Electron microscopical studies and restriction analysis of Helicoverpa armigera nucleo polyhedrosis virus. J Biosci, 24:361-370.
  • Tang P, Zhang H, Li YN, Han B, Wang GZ, Qin QL, Zhang ZF (2012) Genomic sequencing and analyses of HearMNPV-a new multinucleocapsid nucleopolyhedrovirus isolated from Helicoverpa armigera. Virology Journal, 9:168.
  • Tatchell GM (1997) Microbial insecticides and IPM: Current and future opportunities for the use of biopesticides. In: Evans HF (ed) Microbial Insecticides: Novelty or Necessity? British Crop Protection Council, Farnham.
  • Tay WT, Soria MF, Walsh T, Thomazoni D, Silvie P, Behere GT, Anderson C, Downes S (2013) A brave New World for an Old World pest: Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil. PLoS One, 8, e80134.
  • Volkman LE, Blissard GW, Friesen PD, Keddie BA, Possee RD, Theilmann DA (1995) Family Baculoviridae. In: Murphy FA, Fauquet CM, Bishop A, Ghabrial AW, Jarvis GP, Martelli MA, Mayo MA, Summers MD (eds) Virus taxonomy: Sixth report of the International Committee on Taxonomy of Viruses, Springer, Vienna.
  • Washburn JO, Chan EY, Volkman LE, Aumiller JJ, Jarvis DL (2003) Early synthesis of budded virus envelope fusion protein GP64 enhances Autographa cClifornica multicapsid nucleopolyhedrovirus virulence in orally infected heliothis virescens. Journal of Virology, 77:280-290.
  • Washburn JO, Lyons EH, Haas-Stapleton EJ, Volkman LE (1999) Multiple nucleocapsid packaging of Autographa Californica nucleopolyhedrovirus accelerates the onset of systemic Infection in Trichoplusia ni. Journal of Virology, 73:411-416.
  • Williams HL, Monge-Monge KS, Otvos IS, Reardon R, Ragenovich I (2011) Genotypic variation among Douglas-fir tussock moth nucleopolyhedrovirus (OpNPV) isolates in the Western United States. Journal of Invertebrate Pathology, 108:13-21.
  • Yang Y, Li Y, Wu Y (2013) Current status of insecticide resistance in Helicoverpa armigera after 15 years of Bt cotton planting in China. Journal of Economic Entomology, 106: 375-381.

Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye

Year 2023, Volume: 24 Issue: 1, 187 - 195, 15.05.2023
https://doi.org/10.17474/artvinofd.1270318

Abstract

The cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is a major pest of agricultural crops, such as cotton, horticultural plants, a range of vegetable crops and forest trees. Many dead and live larvae and pupa samples of H. armigera, an extremely polyphagous pest, were collected from Adana in Türkiye. It was observed that cadaveric integuments were fragile, and their body tissues were also liquefied. Studies with phase-contrast microscopy revealed a very intense baculovirus infection in cadavers. Transmission electron microscopy exhibited that the new baculovirus had one nucleocapsid in an envelope in the occlusion bodies. Therefore, the isolate was named Helicoverpa armigera single nucleopolyhedrovirus-D (HearSNPV-D). The phylogenetic analysis according to the polyhedrin and late expression factor-8 genes appeared that the new isolate was in close relationship with HearSNPV China and Türkiye isolate. The new isolate was tested on six different insects including its own host Helicoverpa armigera, Hyphantria cunea, Malacosoma neustria, Lymantria dispar, Thaumetopea pityocampa and Spodoptera exigua larvae. Bioassay studies showed that HearSNPV-D was virulent to H. armigera and had a narrow host range. The LC50 value was determined as 0.7 × 105 and 1.9 × 107 OBs/ml for H. armigera and S. exigua, respectively. Non-significant mortality was observed in non-host species by HearSNPV-D, which may reveal the highly specific nature of HearSNPV-D. Therefore, HearSNPV-D can be used as a specific, environmentally friendly control agent for H. armigera.

References

  • Abid AD, Saeed S, Zaka SM, Shahzad S, Naqqash MN, Iqbal N (2022) Host range and pathogenicity potential of Helicoverpa armigera nucleopolyhedrovirus (hanpv) to lepidopterous pests of cotton. Journal of King Saud University - Science, 34 (2), Art. No. 101740.
  • Adams JR, Mcclintock JT (1991) Baculoviridae. Nuclear Polyhedrosis Viruses. In: Adams JR, Bonami JR (eds) Atlas of Invertebrate Viruses, Boca Raton, Florida, CRC Press, pp 87-204.
  • Bergomaz B, Boppre´ M (1986) A simple insect diet for rearing Arctiidae and other moths. J Lepid Soc, 40: 131-137.
  • Bilimoria SL (1991) The biology of nuclear polyhedrosis viruses. In: Kurstak E (ed) Viruses of Invertebrates, Marcel Dekker Inc., New York.
  • Black BC, Brennan LA, Dierks LA, Gard IE (1997) Commercialization of baculoviral insecticides. In: Miller LK (ed) The Baculoviruses, Plenum Press, New York.
  • Caballero P, Zuidema D, Santiago-Alvarez C, Vlak JM (1992) Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Sci Technol, 2:145-157. Cherry A, Cock MJW, Van den Berg H, Kfir R (2003) Biological control of Helicoverpa armigera in Africa. In: Neuenschwander P, Borgemeister C, Langewald. J. (eds) United Kingdom, Wallingford. Cooper D, Cory JS, Theilmann DA, Myers JH (2003) Nucleopolyhedroviruses of forest and western tent caterpillars: cross-infectivity and evidence for activation of latent virus in high-density field populations. Ecological Entomology, 28: 41-50.
  • Cory JS, Green BM, Paul RK, Hunter-Fujita F (2005) Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. J Invertebr Pathol, 89: 101-111.
  • Cory JS, Myers JH (2003) The ecology and evolution of insect baculoviruses. Annual Review of Ecology, Evolution, and Systematics, 34:239-272.
  • Cunningham JC (1998) Insect Viruses and Pest Management. In: Hunter-Fujita FR, Entwistle PF, Evans HF, Crook NE (eds) John Wiley & Sons, Inc., New York.
  • Dias PM, Loureiro ESL, Pessoa LGA, Neto FMO, Tosta RAS, Teodoro PE (2019) Interactions between Fungal-Infected Helicoverpa armigera and the Predator Chrysoperla externa. Insects, 10:309.
  • Eroglu GB, Demir I, Demirbag Z (2018) A novel alphabaculovirus isolated from the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae): characterization and pathogenicity. Biologia, 73: 545-551.
  • Eroglu GB, Nalcacioglu R, Demirbag Z (2019) A new Helicoverpa armigera nucleopolyhedrovirus isolate from Heliothis peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) in Turkey. Turk J Biol, 43:340-348.
  • Federici B (1995) Baculovirus pathogenesis. In: Miller LK (ed) The Baculoviruses, Plenum Press.
  • Ferrelli ML, Taibo C, Fichetti P, Sciocco Cap A, Arneodo JD (2016). Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology, 138: 89–93.
  • Fuxa JR, Richter AR, Srother MS (1993) Detection of Anticarsia gemmatalis nuclear polyhedrosis virus in predatory arthropods and parasitoids after viral release in Louisiana soybeen. Journal of Entomological Science, 28:51-60.
  • Gencer D, Bayramoglu Z, Mutlu C, Demir I (2022) Isolation and characterization of novel Spodoptera exigua nucleopolyhedrovirus strains in Turkey and their potential for use in biological control. Acta Virologica, 66(4):360-368.
  • Gencer D, Bayramoglu Z, Nalcacıoglu R, Kleespies RG, Demirbag Z, Demir I (2018) Characterization of three alphabaculovirus isolates from the gypsy moth, Lymantria dispar dispar (Lepidoptera: Erebidae), in Turkey. Biocontrol Science and Technology, 28 (2):107-121.
  • Haase S, Sciocco-Cap A, Romanowski V (2015) Baculovirus Insecticides in Latin America: Historical Overview, Current Status and Future Perspectives. Viruses, 7:2230-2267.
  • Haile F, Nowatzki T, Storer N (2021) Overview of pest status, po-tential risk, and management considerations of Helicoverpa armigera (Lepidoptera: Noctuidae) for U.S. Soybean Production. Journal of Integrated Pest Management, 12.
  • Hall TA (1999) Bioedit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98.
  • Harrison R, Hoover K (2012) Baculoviruses and other occluded insect viruses. In: Vega FE, Kaya HK (eds) Insect Pathology, Academic Press. Hughes PR, Van Beek NAM, Wood HA (1986) A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctuid larvae. Journal of Invertebrate Pathology, 83: 187-192.
  • Il’inykh AV, Ul’yanova EG (2005) Latency of baculoviruses. Biology Bulletin, 32(5): 496-502.
  • Jacob A, Subramanian TR (1972) Nuclear polyhedrosis on some Lepidoptera. Curr Sci, 41:536.
  • Jehle JA, Lange M, Wang H, Hu Z, Wang Hauschild R (2006) Molecular identification and phylogenetic analysis of baculoviruses from Lepidoptera. Virology, 346:180-193.
  • Kioukia N, Nienow AW, Emery AN, Al-Rubeai M (1995) Physiological and environmental factors affecting the insect cells and infection with baculovirus. Journal of Biotechnology, 38: 243-251.
  • Kumar CS, Ranga Rao GV, Sireesha K, Kumar PL (2011) Isolation and characterization of baculoviruses from three major lepidopteran pests in the semiarid tropics of India. Indian Journal of Virology, 22:29-36.
  • Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution, 33, 1870-1874.
  • Luttrell RG, Jackson RE (2012) Helicoverpa zea and Bt cotton in the United States. GM. Crops, 3: 213–227.
  • Mardani-Talaee M, Nouri-Ganbalani G, Naseri B, Hassanpour M, Mottaghinia L, Mansouri S (2016) Comprative life table parameters of the Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) on corn hybrids under laboratory conditions. J Entomol Soc Iran, 35(4):17-27.
  • Martin T, Ochou GO, Djihinto A, Traore D, Togola M, Vassal JM, Vaissayre M, Fournier D (2005) Controlling an insecticide-resistant bollworm in West Africa. Agric Ecosystems Environ, 107(4): 409-411.
  • Milks ML (1997) Comparative biology and susceptibility of cabbage looper (Lepidoptera: Noctuidae) lines to a nuclear polyhedrosis virus. Environ Entomol, 26:839-848.
  • Nakai M, Kunimi Y (1997) Granulosis virus infection of the smaller tea tortrix (Lepidoptera: Tortricidae): Effect on the development of the endoparasitoid, Ascogaster reticulatus (Hymenoptera: Braconidae). Biological Control, 8:74-80.
  • Nawaz A, Ali H, Sufyan M, Gogi MD, Arif MJ, Ranjha MH, Arshid M, Waseem M, Mustafa T, Qasim M, Rizwan M, Zaynab M, Khan KA, Ghramh HA (2019) Comparative bio-efficacy of nuclear polyhedrosis virus (NPV) and Spinosad against American bollwormm, Helicoverpa armigera (Hubner). Revista Brasileira de Entomologia, 63 (4): 277-282.
  • O'Reilly DR, Miller LK, Luckow VA (1992) Baculovirus Expression Vectors. Laboratory Manual, Oxford University Press, New York.
  • Ribeiro HC, Pavan O, Moutri A (1997) Comparative susceptibility of two different hosts to genotypic variants of the Anticarsia gemmatalis nuclear polyhedrosis virus. Entomol Exp Appl, 83: 233-237.
  • Rodríguez VA, Belaich MN, Quintana G, Sciocco-Cap A, Ghiringhelli PD (2012) Isolation and Characterization of a Nucleopolyhedrovirus from Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae), Int J Virol Mol Biol, 1:28-34.
  • Shapiro M, Robertson JL (1991) Natural variability of three geographic isolates of gypsy moth (Lepidoptera: Lymantriidae) nuclear polyhedrosis virus. J Econ Entomol, 84:71-75.
  • Sharma H (2005) Heliothis/Helicoverpa management: Emerging trends and prospects for future research. Oxford& IBH Publishing Co.
  • Somasekar S, Jayapragasam M, Rabindra RJ (1993) Characterization of five Indian isolates of the nuclear polyhedrosis virus of Helicoverpa armigera. Phytoparasitica, 21:333-337.
  • Sudhakar S, Mathavan S (1999) Electron microscopical studies and restriction analysis of Helicoverpa armigera nucleo polyhedrosis virus. J Biosci, 24:361-370.
  • Tang P, Zhang H, Li YN, Han B, Wang GZ, Qin QL, Zhang ZF (2012) Genomic sequencing and analyses of HearMNPV-a new multinucleocapsid nucleopolyhedrovirus isolated from Helicoverpa armigera. Virology Journal, 9:168.
  • Tatchell GM (1997) Microbial insecticides and IPM: Current and future opportunities for the use of biopesticides. In: Evans HF (ed) Microbial Insecticides: Novelty or Necessity? British Crop Protection Council, Farnham.
  • Tay WT, Soria MF, Walsh T, Thomazoni D, Silvie P, Behere GT, Anderson C, Downes S (2013) A brave New World for an Old World pest: Helicoverpa armigera (Lepidoptera: Noctuidae) in Brazil. PLoS One, 8, e80134.
  • Volkman LE, Blissard GW, Friesen PD, Keddie BA, Possee RD, Theilmann DA (1995) Family Baculoviridae. In: Murphy FA, Fauquet CM, Bishop A, Ghabrial AW, Jarvis GP, Martelli MA, Mayo MA, Summers MD (eds) Virus taxonomy: Sixth report of the International Committee on Taxonomy of Viruses, Springer, Vienna.
  • Washburn JO, Chan EY, Volkman LE, Aumiller JJ, Jarvis DL (2003) Early synthesis of budded virus envelope fusion protein GP64 enhances Autographa cClifornica multicapsid nucleopolyhedrovirus virulence in orally infected heliothis virescens. Journal of Virology, 77:280-290.
  • Washburn JO, Lyons EH, Haas-Stapleton EJ, Volkman LE (1999) Multiple nucleocapsid packaging of Autographa Californica nucleopolyhedrovirus accelerates the onset of systemic Infection in Trichoplusia ni. Journal of Virology, 73:411-416.
  • Williams HL, Monge-Monge KS, Otvos IS, Reardon R, Ragenovich I (2011) Genotypic variation among Douglas-fir tussock moth nucleopolyhedrovirus (OpNPV) isolates in the Western United States. Journal of Invertebrate Pathology, 108:13-21.
  • Yang Y, Li Y, Wu Y (2013) Current status of insecticide resistance in Helicoverpa armigera after 15 years of Bt cotton planting in China. Journal of Economic Entomology, 106: 375-381.
There are 48 citations in total.

Details

Primary Language English
Subjects Structural Biology, Environmental Sciences
Journal Section Research Article
Authors

Donus Gencer 0000-0002-5179-3763

Publication Date May 15, 2023
Acceptance Date April 26, 2023
Published in Issue Year 2023Volume: 24 Issue: 1

Cite

APA Gencer, D. (2023). Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 24(1), 187-195. https://doi.org/10.17474/artvinofd.1270318
AMA Gencer D. Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye. ACUJFF. May 2023;24(1):187-195. doi:10.17474/artvinofd.1270318
Chicago Gencer, Donus. “Isolation Characterization and Pathogenicity of Helicoverpa Armigera Single Nucleopolyhedrovirus Isolate from Türkiye”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 24, no. 1 (May 2023): 187-95. https://doi.org/10.17474/artvinofd.1270318.
EndNote Gencer D (May 1, 2023) Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 24 1 187–195.
IEEE D. Gencer, “Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye”, ACUJFF, vol. 24, no. 1, pp. 187–195, 2023, doi: 10.17474/artvinofd.1270318.
ISNAD Gencer, Donus. “Isolation Characterization and Pathogenicity of Helicoverpa Armigera Single Nucleopolyhedrovirus Isolate from Türkiye”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 24/1 (May 2023), 187-195. https://doi.org/10.17474/artvinofd.1270318.
JAMA Gencer D. Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye. ACUJFF. 2023;24:187–195.
MLA Gencer, Donus. “Isolation Characterization and Pathogenicity of Helicoverpa Armigera Single Nucleopolyhedrovirus Isolate from Türkiye”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, vol. 24, no. 1, 2023, pp. 187-95, doi:10.17474/artvinofd.1270318.
Vancouver Gencer D. Isolation characterization and pathogenicity of Helicoverpa armigera single nucleopolyhedrovirus isolate from Türkiye. ACUJFF. 2023;24(1):187-95.
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