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An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey

Year 2016, Volume: 16 Issue: 1, 4 - 18, 17.11.2016

Abstract

In this study antibacterial, antifungal and antioxidant activities of propolis samples from the Rize province of Turkey in different solvents were investigated. A total of fifteen microorganisms belonging to Gram-positive (Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans, Streptococcus pneumoniae, Streptococcus salivarius), Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enteritidis) and a fungi (Candida albicans) were studied using a disc-diffusion and minimal inhibition concentration (MIC) methods. Ethanol extracts of propolis (EEP), acetone extracts of propolis (AEP), ethyl acetate extracts of propolis (EAEP) and methanol extracts of propolis (MEP) showed the highest antimicrobial activity against S. mutans, L. monocytogenes, M. luteus, B. licheniformis and C. albicans. While dimethyl sulfoxide extracts of propolis (DMSOEP) has the weakly activity against some test organism. The most sensitive microorganisms to propolis were E. coli, B. licheniformis, S. mutans, L. monocytogenes and B. cereus in the test microorganisms. Finally, according to the results shown by GC-MS, at least one substance was dissolved in EAEP and also by us, was found to have the highest antioxidant effect in the EAEP and AEP and the highest antimicrobial effect in the AEP

References

  • [1] GHISALBERTI, E L (1979) Propolis: a review. Bee World, 60: 59-84.
  • [2] BURDOCK, G A (1998) Review of the biological properties and toxicity of propolis. Food Chem Toxicol, 36:341-363.
  • [3] BANKOVA, V (2005). Chemical diversity of propolis and problem of standardization. Journal of Ethnopharmacology, 100: 114–117.
  • [4] MATSUNO, T (1997) O efeito terapêutico da propolis. New York: Columbia University, Institute of Cancer Research.
  • [5] SALATINO, A; TEIXEIRA, É W; NEGRI, G; MESSAGE, D (2005) Origin and Chemical Variation of Brazilian Propolis. Evidence-Based Complementary and Alternative Medicine, 2(1): 33-38.
  • [6] CASTALDO, S; CAPASSO, F (2000) Propolis, an old remedy used in modern medicine. Fitoterapia, 73(1): 1-6[7] ADELMANN, J; PASSOS, M; BREYER, D H, ROCHA DOS SANTOS, M H; LENZ, C; LEITE, N F;LANÇAS, F M; FONTANA, J D (2007) Exotic flora dependence of an unusual Brazilian propolis: The pinocembrin biomarker by capillary techniques. Journal of Pharmaceutical and Biomedical Analysis, 43: 174-178.
  • [7] BANKOVA, V; POPOVA, M; BOGDANOV, S; SABATINI, A G (2002) Chemical composition of European propolis: expected and unexpected results. Z Naturforsch, 57: 530-533.
  • [8] MONTENEGRO, G; TIMMERMANN, B N; PENA, R C; MUJICA, A M; AVILA, G (2000) Pollen grains and vegetative structures in propolis as indicators of potential drugs in Chilean plants. Phyton 66: 15–23.
  • [9] PARK, Y K; PAREDES, G J F; AGUIAR, C L; ALENCAR, S M; FUJIWARA, F Y (2004) Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis. The Journal of Agricultural and Food Chemistry, 52(5): 1100–1103.
  • [10] RONALD, M A (1990) Microbiologia, Compania Editorial Continental S.A. de C.V., Mexico, 1 D. F.
  • [11] VANDEN, B D A VLIETINCK, A J (1991) Screening methods for antibacterial and antiviral agents from higger plants. In: DEy, P.M., Harborne, J.B. (Eds.) Methods in Plant Biochemistry.London.
  • [12] POPOVA, M; SILICI, S; KAFTANOĞLU, O; BANKOVA, V (2005) Antibacterial activity of Turkish propolis and Its Qualitative and Quantitative Chemical Composition. Phytomedicine, 12: 221-228.
  • [13] KÜÇÜK, M; KOLAYLI, S; KARAOĞLU, Ş; ULUSOY, E; BALTACI, C; CANDAN, F (2007) Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, 100: 526-534.
  • [14] BENZIE, I F F; STRAIN, J J (1999) Methods in Enzymology Orlando. FL: Academic, 299: 15 27.
  • [15] MARSH, P D (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res, 8: 263–271.
  • [16] LEPEKHIN, V N; LEONOVA, T A (1970) Antimicrobial properties of propolis. Stomatologija, 49(4): 16-19.
  • [17] KOO, H; GOMES, B P F A; ROSALEN, P L; AMBROSANO, G M B; PARK, Y K; CURY, J A (2000) In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol, 45: 141–148.
  • [18] PARK, Y K; KOO, M H; ABREU, J A S; IKEGAKI, M; CURY, J A; ROSALEN, P L (1998) Antimicrobial Activity of Propolis on Oral microorganisms. Current Microbiology, 36: 24-28.
  • [19] UGUR, A; ARSLAN, T (2004) An In Vitro Study on Antimicrobial Activity of Propolis from Mugla Province of Turkey. Journal of Medicinal Food, 7(1): 90-94.
  • [20] HEGAZI, A G; FATEN, K; FAYROUZ, A M; ABD, A (2000) Chemical Composition and Antimicrobial Activity of European Propolis. Z Naturforsch, 55: 70-75.
  • [21] [BANKOVA, V; CHRISTOV, R; KUJUMGIEV, A; MARCUCCI, M C; POPOV, S (1995) Chemical composition and antibacterial activity of Brazilian propolis. Z Naturforsch, 50: 167-172.
  • [22] [BANSKOTA, A H; TEZUKA, Y; PRASAIN, J K; MATSUSHIGE, K; SAIKI, I; KADOTA, S (1998) Chemical constituents of Brazilian propolis and their cytotoxic activities. J Nat Prod, 61: 896-900.
  • [23] TAZAWA, S; WARASHINA, T; NORO, T; MIYASE, T (1998) Studies on the constituents of Brazilian propolis. Chem Pharm Bull, 46: 1477-1479.
  • [24] AMOROS, M; SIMÕES, C M; GIRRE, L; SAUVAGER, F; CORMIER, M (1992) Synergistic effect of flavones and flavonols against herpes simplex virus type 1 in cell culture. Comparison with the antiviral activity of propolis. Journal of Natural. Products, 55(12): 1732-1740.
  • [25] BONVEHI, J S; COLL, F V; JORDÀ, R E (1994) The composition, active components and bacteriostatic activity of propolis in dietetics. J Am Oil Chem Soc, 71: 529-532.
  • [26] GÉLINAS, P; MCKINNON, C M (2006) Effect of wheat variety, farming site, and bread-baking on total phenolics. International Journal of Food Science and Technology, 41(3): 329-332.
  • [27] GREGORIS, E; STEVANATO, R (2010) Correlations between polyphenolic composition and antioxidant activity of Venetian propolis. Food and Chemical Toxicology, 48: 76-82.
  • [28] AHN, M R; KUMAZAWA, S; USUİ, Y; NAKAMURA, J; MATSUKA, M; ZHU, F; NAKAYAMA, T (2007) Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry, 10: 1383-1392.
  • [29] KUJUMGIEV, A; BANKOVA, V; IGANTOVA, A; POPOV, S (1993) Antibacterial activity of propolis, some of its components and their analogs. Pharmazie, 48(10): 785-786.
  • [30] MARCUCCİ, M C; FERRERES, F; GARCÍA, V C; BANKOVA, V S; DE CASTRO, S L; DANTAS, A P; VALENTE, P H M; PAULİNO, N (2001) Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology, 74; 105–112.
  • [31] KARTAL, M; YILDIZ, S; KAYA, S; KURUCU, S; TOPCU, G (2003) Antimicrobial activity of propolis samples from two different regions. Anatolia J Ethnopharmacol, 86: 69-73.

Türkiye’de Rize İlinden Elde Edilen Propolisin Antimikrobiyal ve Antioksidan Aktivitesi Üzerine İn Vitro Çalışma

Year 2016, Volume: 16 Issue: 1, 4 - 18, 17.11.2016

Abstract

Bu çalışmada Türkiye’nin Rize ilinden toplanan propolis örneklerinin farklı solventlerde antibakteriyal antifungal ve antioksidan aktiviteleri araştırılmıştır.Gram pozitif bakteriler (Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Listeria monocytogenes, Micrococcus luteus, Staphylococcus aureus, Streptococcus mutans, Streptococcus pneumoniae, Streptococcus salivarius), Gram negatif bakteriler (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enteritidis) ve bir maya  (Candida albicansolmak üzere toplamda 15 mikroorganizma disk difüzyon ve minimum inhibisyon konsantrasyonu (MİK) yöntemi  kullanılarak incelenmiştir. Propolisin etanol ekstraktı (EPE), propolisin aseton ekstraktı (APE), propolisin etil asetat ekstraktı (EAPE) ve propolisin metanol ekstraktı (MPE) S. mutans, L. monocytogenesis, M. luteus, B. licheniformis ve C. albicans’a karşı en yüksek antimikrobiyal aktivite göstermiştir. Dimetil sülfoksit Propolis Ekstraktı (DMSOPE) bazı test mikroorganizmalarına karşı zayıf aktivite göstermiştir. Propolise en duyarlı mikroorganizmalar E. coli, B. licheniformis, S. mutans, L. monocytogenes ve B. cereus olmuştur.

References

  • [1] GHISALBERTI, E L (1979) Propolis: a review. Bee World, 60: 59-84.
  • [2] BURDOCK, G A (1998) Review of the biological properties and toxicity of propolis. Food Chem Toxicol, 36:341-363.
  • [3] BANKOVA, V (2005). Chemical diversity of propolis and problem of standardization. Journal of Ethnopharmacology, 100: 114–117.
  • [4] MATSUNO, T (1997) O efeito terapêutico da propolis. New York: Columbia University, Institute of Cancer Research.
  • [5] SALATINO, A; TEIXEIRA, É W; NEGRI, G; MESSAGE, D (2005) Origin and Chemical Variation of Brazilian Propolis. Evidence-Based Complementary and Alternative Medicine, 2(1): 33-38.
  • [6] CASTALDO, S; CAPASSO, F (2000) Propolis, an old remedy used in modern medicine. Fitoterapia, 73(1): 1-6[7] ADELMANN, J; PASSOS, M; BREYER, D H, ROCHA DOS SANTOS, M H; LENZ, C; LEITE, N F;LANÇAS, F M; FONTANA, J D (2007) Exotic flora dependence of an unusual Brazilian propolis: The pinocembrin biomarker by capillary techniques. Journal of Pharmaceutical and Biomedical Analysis, 43: 174-178.
  • [7] BANKOVA, V; POPOVA, M; BOGDANOV, S; SABATINI, A G (2002) Chemical composition of European propolis: expected and unexpected results. Z Naturforsch, 57: 530-533.
  • [8] MONTENEGRO, G; TIMMERMANN, B N; PENA, R C; MUJICA, A M; AVILA, G (2000) Pollen grains and vegetative structures in propolis as indicators of potential drugs in Chilean plants. Phyton 66: 15–23.
  • [9] PARK, Y K; PAREDES, G J F; AGUIAR, C L; ALENCAR, S M; FUJIWARA, F Y (2004) Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis. The Journal of Agricultural and Food Chemistry, 52(5): 1100–1103.
  • [10] RONALD, M A (1990) Microbiologia, Compania Editorial Continental S.A. de C.V., Mexico, 1 D. F.
  • [11] VANDEN, B D A VLIETINCK, A J (1991) Screening methods for antibacterial and antiviral agents from higger plants. In: DEy, P.M., Harborne, J.B. (Eds.) Methods in Plant Biochemistry.London.
  • [12] POPOVA, M; SILICI, S; KAFTANOĞLU, O; BANKOVA, V (2005) Antibacterial activity of Turkish propolis and Its Qualitative and Quantitative Chemical Composition. Phytomedicine, 12: 221-228.
  • [13] KÜÇÜK, M; KOLAYLI, S; KARAOĞLU, Ş; ULUSOY, E; BALTACI, C; CANDAN, F (2007) Biological activities and chemical composition of three honeys of different types from Anatolia. Food Chemistry, 100: 526-534.
  • [14] BENZIE, I F F; STRAIN, J J (1999) Methods in Enzymology Orlando. FL: Academic, 299: 15 27.
  • [15] MARSH, P D (1994) Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res, 8: 263–271.
  • [16] LEPEKHIN, V N; LEONOVA, T A (1970) Antimicrobial properties of propolis. Stomatologija, 49(4): 16-19.
  • [17] KOO, H; GOMES, B P F A; ROSALEN, P L; AMBROSANO, G M B; PARK, Y K; CURY, J A (2000) In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol, 45: 141–148.
  • [18] PARK, Y K; KOO, M H; ABREU, J A S; IKEGAKI, M; CURY, J A; ROSALEN, P L (1998) Antimicrobial Activity of Propolis on Oral microorganisms. Current Microbiology, 36: 24-28.
  • [19] UGUR, A; ARSLAN, T (2004) An In Vitro Study on Antimicrobial Activity of Propolis from Mugla Province of Turkey. Journal of Medicinal Food, 7(1): 90-94.
  • [20] HEGAZI, A G; FATEN, K; FAYROUZ, A M; ABD, A (2000) Chemical Composition and Antimicrobial Activity of European Propolis. Z Naturforsch, 55: 70-75.
  • [21] [BANKOVA, V; CHRISTOV, R; KUJUMGIEV, A; MARCUCCI, M C; POPOV, S (1995) Chemical composition and antibacterial activity of Brazilian propolis. Z Naturforsch, 50: 167-172.
  • [22] [BANSKOTA, A H; TEZUKA, Y; PRASAIN, J K; MATSUSHIGE, K; SAIKI, I; KADOTA, S (1998) Chemical constituents of Brazilian propolis and their cytotoxic activities. J Nat Prod, 61: 896-900.
  • [23] TAZAWA, S; WARASHINA, T; NORO, T; MIYASE, T (1998) Studies on the constituents of Brazilian propolis. Chem Pharm Bull, 46: 1477-1479.
  • [24] AMOROS, M; SIMÕES, C M; GIRRE, L; SAUVAGER, F; CORMIER, M (1992) Synergistic effect of flavones and flavonols against herpes simplex virus type 1 in cell culture. Comparison with the antiviral activity of propolis. Journal of Natural. Products, 55(12): 1732-1740.
  • [25] BONVEHI, J S; COLL, F V; JORDÀ, R E (1994) The composition, active components and bacteriostatic activity of propolis in dietetics. J Am Oil Chem Soc, 71: 529-532.
  • [26] GÉLINAS, P; MCKINNON, C M (2006) Effect of wheat variety, farming site, and bread-baking on total phenolics. International Journal of Food Science and Technology, 41(3): 329-332.
  • [27] GREGORIS, E; STEVANATO, R (2010) Correlations between polyphenolic composition and antioxidant activity of Venetian propolis. Food and Chemical Toxicology, 48: 76-82.
  • [28] AHN, M R; KUMAZAWA, S; USUİ, Y; NAKAMURA, J; MATSUKA, M; ZHU, F; NAKAYAMA, T (2007) Antioxidant activity and constituents of propolis collected in various areas of China. Food Chemistry, 10: 1383-1392.
  • [29] KUJUMGIEV, A; BANKOVA, V; IGANTOVA, A; POPOV, S (1993) Antibacterial activity of propolis, some of its components and their analogs. Pharmazie, 48(10): 785-786.
  • [30] MARCUCCİ, M C; FERRERES, F; GARCÍA, V C; BANKOVA, V S; DE CASTRO, S L; DANTAS, A P; VALENTE, P H M; PAULİNO, N (2001) Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology, 74; 105–112.
  • [31] KARTAL, M; YILDIZ, S; KAYA, S; KURUCU, S; TOPCU, G (2003) Antimicrobial activity of propolis samples from two different regions. Anatolia J Ethnopharmacol, 86: 69-73.
There are 31 citations in total.

Details

Subjects Structural Biology, Zootechny (Other), Veterinary Surgery
Journal Section Articles
Authors

Ömer Ertürk

Elif Çil This is me

Nihal Yoloğlu This is me

Ceren Yavuz This is me

Publication Date November 17, 2016
Submission Date July 10, 2015
Acceptance Date July 22, 2016
Published in Issue Year 2016 Volume: 16 Issue: 1

Cite

APA Ertürk, Ö., Çil, E., Yoloğlu, N., Yavuz, C. (2016). An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera, 16(1), 4-18.
AMA Ertürk Ö, Çil E, Yoloğlu N, Yavuz C. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. mellifera. November 2016;16(1):4-18.
Chicago Ertürk, Ömer, Elif Çil, Nihal Yoloğlu, and Ceren Yavuz. “An In Vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey”. Mellifera 16, no. 1 (November 2016): 4-18.
EndNote Ertürk Ö, Çil E, Yoloğlu N, Yavuz C (November 1, 2016) An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera 16 1 4–18.
IEEE Ö. Ertürk, E. Çil, N. Yoloğlu, and C. Yavuz, “An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey”, mellifera, vol. 16, no. 1, pp. 4–18, 2016.
ISNAD Ertürk, Ömer et al. “An In Vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey”. Mellifera 16/1 (November 2016), 4-18.
JAMA Ertürk Ö, Çil E, Yoloğlu N, Yavuz C. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. mellifera. 2016;16:4–18.
MLA Ertürk, Ömer et al. “An In Vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey”. Mellifera, vol. 16, no. 1, 2016, pp. 4-18.
Vancouver Ertürk Ö, Çil E, Yoloğlu N, Yavuz C. An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. mellifera. 2016;16(1):4-18.