Cilt 14, Sayı 1, Sayfalar 94 - 103 2012-11-11

Against Drought Stress Effect of Antioxidant Enzymes of Boron
Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi

Mahmut Doğan [1] , Aslıhan AVU [2]

169 388

In this study, soybean seeds (Glycine max. L., cv., “A3935) were grown under controlled conditions (25±2 C) composed of different boron compounds. In the experiment, 5 groups were determined respectively as potassium tetraborate tetrahydrate (1 mg/1), ammonium tetraborate tetrahydrate (1 mg/1), sodium boron hydride (1 mg/1), lithium tetraborate tetrahydrate (100 mg/1), and sodium tetraborate decahydrate (100 mg/1). The doses used in this study were determined according to the results of a preliminary study. Soybean seeds were exposed to different amounts of drought stress based on time (control, 3, 6, 9, 12, 15, and 18 days). Activities of antioxidant enzymes superoxide dismutase (SOD: EC 1.15.1.1), glutathione reductase (GR: EC 1.6.4.2), ascorbate peroxidase (APX: EC 1.11.1.11) and catalase (CAT: EC 1.11.1.6) measured. According to the results stress+potassium tetraborate tetrahydrate environment has increased the amount of CAT, decreased the amount GR, APX and SOD. Potassium tetraborate 0.1 mg / l dose administration is the most appropriate critical value, and the most important indicator of drought CAT enzyme found to give the best results.
Bu çalışmada materyal olarak soya (Glycine max. L., cv., “A3935”) tohumları farklı bor bileşiklerinden oluşan, kontrollü koşullarda (25+2 C) yetiştirilmiştir. Araştırmada kontrol grubuna herhangi bir bor uygulaması yapılmamıştır. Diğer uygulamalar sırasıyla; potasyum tetraborat tetrahidrattan 0.1 mg/l, amonyum tetraborat tetrahidrattan 1 mg/l, sodyum bor hidrürden 1 mg/l, lityum tetraborat tetrahidrattan 100 mg/l, sodyum tetraborat dekahidrattan 100 mg/l olmak üzere belirlenen uygun doz kullanılmıştır. Araştırma tesadüf parselleri deneme desenine göre planlanmıştır. Soya fideleri zamana bağlı olarak farklı kuraklık (kontrol, 3, 6, 9, 12, 15 ve 18 gün) uygulamalarına maruz bırakılmıştır. Yapraklarda katalaz (CAT; EC= 1.11.1.6), gulutatyon redüktaz (GR; EC= 1.6.4.2), askorbat peroksidaz (APX; EC= 1.11.1.11) ve süperoksit dismutaz (SOD; EC= 1.15.1.1) enzim aktiviteleri ölçülmüştür. Analiz sonuçlarına göre Stres+potasyum tetraborat tetrahidrat ortamında CAT miktarı artmış, GR, APX ve SOD miktarı azalmıştır. Potasyum tetraboratın 0.1 mg/l doz uygulamasının en uygun eşik değeri olduğu ve kuraklığa karşı en önemli gösterge olan CAT enziminde en iyi sonucu verdiği anlaşılmıştır.
  • Akçam Oluk E, Demiray H (2004) Bor elementinin sambro no: 3 Ayçiçeği ( Helianthus annuus L.) Çeşidinin Büyümesi Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Dergisi, 41(1),1811
  • Akçam Oluk E, Demiray H, Yardım D (2006) Bor Fazlalığının Ayçiçeği ( Helianthus annuus L.cv.Sambro No.5) Bitkisinin İn Vitro Koşullarda Kök Gelişimi ve Anatomisi Üzerine Etkileri. Ege Üniversitesi Ziraat Fakültesi Dergisi, 43(2), 145-152.
  • Ahmadi A, Emam Y, Pessarakli M (2010) Biochemıcal Changes in Maize Seedlings Exposed to Drought Stress Conditions at Different Nitrogen Levels. Journal of Plant Nutrition , 33 (4), 541-556.
  • Asada K (1992) Ascorbate perokxidase a hydrogen peroxide- scavenging enzyme in plants. Physiology Plantarum, 85, 235-241.
  • Ashraf M, Iram A (2005) Drought Stress Induced Changes in Some Organic Substances in Nodules and Other Plant Parts of Two Potential Legumes Differing in Salt Tolerance. Flora, 200, 535–546.
  • Ashraf M, Ali Q (2007) Relative Membrane Permeability and Activities of Some Antioxidant Enzymes as the Key Determinants of Salt Tolerance ın Canola (Brassica Napus L.). Envionmental and Experimental Botany, 63, 266-2
  • Atalay E, Gezgin S, Babaoğlu M (2003) Buğday ( Triticum durum Desf.) ve Arpa ( Hordeum Vulgare L.) İn Vitro Fidelerinin Bor Alımının ICP-AES ile Tespiti. S.Ü. Ziraat Fakültesi Dergisi 17 (32), 47 -52.
  • Bandeoglu E, Eyidogan F, Yucel M, Oktem HA (2004) Antioxidant responses of shoots and roots of lentil to NaCl-salinity stres. Plant Growth Regulation 42, 69–77.
  • Bowler C, Van montagu M, Inze D (1992) Superoxide dismutase and stres tolerance. Annual Review of Plant Physiology Plant Molecular Biology, 43, 83-116.
  • Bruggemann W, Beyel V, Brodka N, Poth H Weil M, Stockhaus J (1999) Antioxidative and antioksidative enzymes in wild-type and transgenic Lycopersicon gentoypes of different chilling tolerance. Plant Science, 140(2), 1451
  • Bishnoi SK, Kumar BN, Rani C, Data S, Kumari P, Sheoran S, Angrish R (2005) Changes in Protein Profile of Pigeonpea Genotypes in Response to NaCl and Boron Stres. Biologia Plantarum, 50(1), 135-137.
  • Choi E, Kolesik P, McNeill A, Collins H, Zhang Q, Huynhi B, Graham R, Stangoulis J (2007) The Mechanism of Boron Tolerance for Maintanance of Root Growth in Barley ( Hordeum vulgare L.). Plant Cell and Environment , 30, 984-993.
  • Chattopadhayay MK, Tiwari BS, Chattopadhayay G, Bose A, Sengupta DN, Ghosh B (2002) Protect role of exogenous polyamines on saliniyt-stressed rice ( Oryza sativa ). Plants, 116, 192-1
  • Çakmak I, Marschner H (1992) Magnesium defficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology, 98, 1222-1226.
  • Çakmak I (1994) Activity of ascorbate-dependent H 2 O 2 -scaveninig enzymes and leaf shlorosis are enhanced in magnesium and potassiumdeficient leaves, but not in phosphorus-deficient leaves. Journal of Experimental Botany, 45, 1259-1266.
  • Daşgan HY, Koç S (2009a) Evaluation of Salt Tolerance in Common Bean Genotypes by Ion Regulation and Searching for Screening Parameters. Journal of Food Agriculture Environment, 7(2), 363-372.
  • Daşgan HY, Kuşvuran Ş, Abak K, Leport L, Larher F, Bouchereau A (2009b) The Relationship Between Citrulline Accumulation and Salt Tolerance During the Vegetative Growth of Melon ( Cucumis melo L.). Plant Soil Envıronment, 55 (2), 51-57.
  • Dalal M, Khanna-Chopra R (2001) Differential response of antioxidant enzymes in leaves of necrotic wheat hybrids and their parents. Physiologia Plantarum, 111, 297-304.
  • Doğan M, Tıpırdamaz R, Demir Y (2010a) Effective salt criteria in callus- cultured tomato genotypes. A Journal of Bioscience Contens, 65, 613- 618.
  • Doğan M, Tıpırdamaz R, Demir Y (2010b) Salt resistance of tomato species grown in sand culture, Plant Soil Environ, 56, 499-507.
  • Doğan M (2011) Antioxidative and proline potentials as a protective mechanism in soybean plants under salinity stres. Africa Journal of Biotechnology , 10(32), 5972-5978.
  • Doğan M (2012) Farklı Bor Uygulamalarının Capparis L. spp. ve Carthamus L. spp. Tohumlarının Çimlenmesi Üzerine Etkisi. Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 16-2, 154-161
  • El-saht HM (1998) Responses to chilling stres in frech bean seedlings: Antioxidant compounds. Biologia Plantarum, 41(3), 395-402.
  • Elstner EF (1982) Oxygen activation and oxygen toxicity. Annual Review of Plant Physiology Plant, 33, 73-96.
  • Fadzillah NM, Gilli V, Finch RP, Burdon RH (1996) Chilling oxidative stres and antoxidant responses in shoot culture of rice, Planta, 199, 552-5
  • Gaspar T, Penel C, Castillo JF, Greppin H (1985) A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiologya Plantarum, 64, 4184
  • Hernandez JA, Almansa MS (2002) Short-term effects salt stres on antioxidant systems and leaf water relations of pea leaves. Physiolgya Plantarum , 115, 251-257.
  • Hoagland DR, Arnon DI (1938) The water culture method for growing plants without Soil. Circ. Calif. Agr. Exp. Sta. , 347-461, Jaleel CA (2009) Non-Enzymatic Antioxidant Changes in Withania Somnifera With Varying Drought Stress Levels. American-Eurasian Journal of Scientific Research, 4(2), 64-67.
  • Jung S (2004) Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Science , 166, 459-466.
  • Jackson C, Dench J, Morore AL, Halliwell B, Foyer CH, Hall DO (1978) Subcellular localisation and identification of superoxide dismutase in the leaves of higher plants. Europan Journal of Biochemstry, 91, 339-344.
  • Kalefetoğlu T, Ekmekçi Y (2005) The effects of drought on plants and tolerance mechanisms (Review).
  • Gazi Üniversitesi, Fen Bilimleri Dergisi, 18(4), 723-740. Karabal E, Yücel M, Hüseyin AÖ (2003) Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity. Plant Science 164, 925–933.
  • Khanna-Chopra R, Selote DS (2007) Acclimation to Drought Stres Generates Oxidative Stress Tolerance in Drought Resistant thanSusceptible Wheat Cultivar Under Field Conditions. Environmental and Experimental Botany, 60, 276–283.
  • Kubo A, Aono M, Nakajima N, Saji H, Tanaka K, Kondo N (1999) Differential responses in activity of antioxidant enzymes to different environmental streses in Arabidopsis thaliana . Journal of Plant Research, 112(3), 279-290.
  • Lahet JJ, Lenfant F, Courderot-Masuyer C, Ecarnot-Laubriet E, Vergely C, DurnetArcheray MJ, Freysz M, Rochette L (2003) In vivo and in vitro antioxidant properties of furosemide. Life Sciences, 73(8), 1075-1082.
  • Lichtenhaler HK (1996) Vegetation stress: an introduction to the stress concept in plants. Journal of Plant Physiology, 148, 4-14
  • Lee DH, Lee CB (2000) Chilling stres-induced shanges of antioxidant enzymes in the leaves of cucumber in gel enzyme activity assays. Plant sciences, 159, 75-85.
  • Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress, Environmet of Experimental Botany, 49, 69–76.
  • Mittova V, Tal M, Volokitta M, Guy M (2002) Salt stres induces up-regulation of an efficient chloroplast antioxidant system in the salttolerant wild tomato species Lycopersicon pennellii but not in the cultivated species. Physiologia Pantarum, 115, 393-400.
  • Mittova V, Guy M, Tal M, Volokita M (2004) Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennelli . Journal of Experimental Botany, 55, 1105- 1113.
  • Munne-Bosch S, Penuelas J (2003) Photo-and antioxidative protection during summer leaf senescence in Pistacia lentiscus L. Grown under mediterranean field conditions. Annals of Botany, 92, 385-391.
  • Nader B, Jimenez A, Megdiche W, Lundqvist M, Sevilla F, Abdelly C (2006) Response of antioxidant systems to NaCl stres in the halophyte Cakile maritima Physiologia Plantarum , 126, 446-457.
  • Oidaire H, Sano S, Koshiba T, Ushimaru T (2000) Enhancement of antioxidative enzyme activities in chilled rice seedlings. Journal of Plant Physiolgy, 156, 811-813.
  • Pinheiro HA, DaMatta FM, Chaves ARM, Fontes EPB, Loureiro ME (2004) Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to longterm drought, Plant Science, 167, 1307-1314.
  • Prasad TK (1997) Role of catalase in inducing chilling tolerance in preemergent miaze seedlings. Plant Physiology, 114, 1369-1376.
  • Ramachandra Reddy A, Chaitanya KV, Jutur PP, Sumithra K (2004) Differential antioxidative responses to water stress among five mulberry ( Morus alba L.) cultivars, Environmet of Experimental Botany, 52, 33-42.
  • Ronald PC, Brown PH (2003) Transgenically enhanced sorbitol synthesis facilitates phloemboron mobility in rice, Physiology Plantarum, 117, 79-84.
  • Scebba F, Sebastiani L, Vitagliano C (1998) Changes in activity of antioxidative enzymes in wheat ( Triticum aestivum ) seedlings under cold acclimation. Physiology Plantarum, 104: 7477
  • Shalata A, Mittova V, Volokita Guy M, Tal M (2001) Response of the cultivated tomato and its wild salt-tolerant relative
  • Lycopersicon pennelli to salt-dependet oxidative stres: The root antioxidative system, Physiologia Plantarum, 112, 487-494. Walker MA, Mckersie BD (1993) Role of the ascorbat- glutathione antioxidant system in chilling resistance of tomato. Journal of Plant Physiology, 14, 234-239.
  • Warington K (1923) The Effects of Boric Acid and Borax on The Broad Bean And Certain Other Plants, Annual of Botany, 37, 457-466.
Birincil Dil tr
Konular
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Mahmut Doğan
E-posta: dogan@harran.edu.tr

Yazar: Aslıhan AVU
E-posta: avu@harran.edu.tr

Bibtex @ { artvinofd29871, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2012}, volume = {14}, pages = {94 - 103}, doi = {}, title = {Against Drought Stress Effect of Antioxidant Enzymes of Boron}, language = {en}, key = {cite}, author = {Doğan, Mahmut and AVU, Aslıhan} } @ { artvinofd29871, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2012}, volume = {14}, pages = {94 - 103}, doi = {}, title = {Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi}, language = {tr}, key = {cite}, author = {Doğan, Mahmut and AVU, Aslıhan} }
APA Doğan, M , AVU, A . (2012). Against Drought Stress Effect of Antioxidant Enzymes of Boron. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 14 (1), 94-103. Retrieved from http://ofd.artvin.edu.tr/issue/2266/29871
MLA Doğan, M , AVU, A . "Against Drought Stress Effect of Antioxidant Enzymes of Boron". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 14 (2012): 94-103 <http://ofd.artvin.edu.tr/issue/2266/29871>
Chicago Doğan, M , AVU, A . "Against Drought Stress Effect of Antioxidant Enzymes of Boron". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 14 (2012): 94-103
RIS TY - JOUR T1 - Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi AU - Mahmut Doğan , Aslıhan AVU Y1 - 2012 PY - 2012 N1 - DO - T2 - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JF - Journal JO - JOR SP - 94 EP - 103 VL - 14 IS - 1 SN - 2146-1880-2146-698X M3 - UR - Y2 - 2017 ER -
EndNote %0 Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi %A Mahmut Doğan , Aslıhan AVU %T Kuraklık Stresine Karşı Borun Antioksidant Enzimlere Etkisi %D 2012 %J Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi %P 2146-1880-2146-698X %V 14 %N 1 %R %U