BibTex RIS Kaynak Göster

Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Yıl 2014, Cilt: 15 Sayı: 2, 149 - 160, 16.01.2015
https://doi.org/10.17474/acuofd.05189

Öz

Genetic diversity in natural stands of autochthonous blackthorn (Prunus spinosa L.) of different German provenances has been analyzed using a highly reproducible high-annealing-temperature random amplified polymorphic DNA (HAT-RAPD) protocol. The findings were compared to those from seedstocks of the same provenances, reported earlier. Generally, genetic diversity in the natural stands was even lower (Ho 0.099–0.116) compared to the corresponding seedstocks (Ho 0.118–0.133). Furthermore, genetic differentiation was found to be moderate between natural residential sources (pairwise Fst 0.138–0.184, 22.527% variation among populations), but higher than between the seedstocks (pairwise Fst 0.086-0.104, 7.782% variation among populations). The findings are discussed in respect to German conservation law and its practical implementation.  

Kaynakça

  • Antao T, Lopes A, Lopes RJ, Beja-Pereira A, Luikart, G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9: 323
  • (Bundesministerium BMU für Umwelt,
  • Naturschutz und Reaktorsicherheit) (2012)
  • Leitfaden zur Verwendung gebietseigener
  • Gehölze. Selbstverlag, Berlin
  • BMVEL (Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft) (2003) Verwendung einheimischer Gehölze regionaler Herkunft für die freie Landschaft. Ein Beitrag zur Erhaltung und Förderung der biologischen Vielfalt. Selbstverlag, Bonn
  • BnatSchG (Gesetz über Naturschutz und Landschaftspflege) Bundesnaturschutzgesetz vom 29.07.2009, in Kraft getreten am 01.03.2010. BGBl I:2542
  • Convention on biological diversity (1992) Secretariat of the convention on biological diversity. http://www.cbd.int/programmes/outreach/aware ness/publications.shtml. Accessed 18th August 2011
  • Crawford KM, Whitney KD (2010) Population genetic diversity influences colonization success. Mol Ecol 19(6):1253–1263
  • Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297-302
  • Eimert K, Rückert F-E, Schröder M.B. (2011) Genetic diversity within and between seedstock populations of several German autochthonous provenances and conventionally propagated nursery material of blackthorn (Prunus spinosa L.). Plant Syst Evol 298(3):609–618
  • Esquinas-Alcázar J (2005) Science and society: protecting crop genetic diversity for food security: political, ethical and technical challenges. Nat Rev Genet 6(12):946–953
  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14(8):2611–2620
  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47–50
  • Fronia R (2009) Prüfung der Identität und Variabilität gebietsfremder Herkünfte von Prunus spinosa L. und Cornus sanguinea L. zur Verwendung in der freien Landschaft. Der Andere Verlag, Tönning, Lübeck, Marburg und Google (2014) Google Earth. Available: http://www.google.com/earth
  • Guitián J, Guitián P, Sánchez JM (1993) Reproductive biology of two Prunus species (Rosaceae) in the Northwest Iberian Peninsula. Plant Syst Evol 185(3-4):153–165
  • Jensen JL, Bohonak AJ, Kelley ST (2005) Isolation by distance, web service. BMC Genet 6(1):13
  • Leinemann L, Bendixen K, Kownatzki D, Hattemer HH, Liepe K, Stenger G (2002) Genetische Untersuchungen an Landschaftsgehölzen im Hinblick auf die Erzeugung und Zertifizierung von Vermehrungsgut. Allgemeine Forst- und Jagdzeitung 173:146-152
  • Leinemann L, Kleinschmit J, Fussi B, Hosius B, Kuchma O, Arenhövel W, Finkeldey R (2014) Genetic composition and differentiation of sloe (Prunus spinosa L.) populations in Germany with respect to the tracing of reproductive plant material. Plant Syst Evol. doi:10.1007/s00606- 014-1027-7
  • Vander Mijnsbrugge K (2013) Genetic and morphological variability among autochthonous Prunus spinosa populations in Flanders (northern part of Belgium): implications for seed sourcing. Plant Ecol Evol 146(2):193–202
  • Mohanty A, Martín JP, Aguinagalde I (2000) Chloroplast DNA diversity within and among populations of the allotetraploid Prunus spinosa L. Theor Appl Genet 100(8):1304–1310
  • Mohanty A, Martín JP, Aguinagalde I (2002) Population genetic analysis of European Prunus spinosa (Rosaceae) using chloroplast DNA markers. Am J Bot 89(8):1223–1228
  • Reynders-Aloisi S, Grellet F (1994) Characterization of the ribosomal DNA units in two related Prunus species (P. Cerasifera and P. Spinosa). Plant Cell Rep 13(11):641–646
  • Hoisington D, Khairallah M, Reeves T, Ribaut J-M, Skovmand B, Taba S, Warburton M (1999) Plant genetic resources: What can they contribute toward increased crop productivity? P Natl Acad Sci USA 96(11):5937–5943
  • Jaccard P (1901) Distribution de la flore alpine dans le bassin des Dranses et dans quelques régions voisines. Bull Soc Vaud Sci Nat 37:241–272
  • Karg S, Markle T (2002) Continuity and changes in plant resources during the Neolithic period in western Switzerland. Veg Hist Archaeobot 11:169–176
  • Karnitsch P (1953) Fundberichte im Jahrbuch der Stadt Linz 1951. Pro Austria Romana 3:26
  • Kimura, M (1968) Evolutionary rate at the molecular level. Nature 217(5129):624–626
  • Kremer A, Kleinschmit J, Cottrell J, Cundall EP, Deans JD, Ducousso A, Stephan BR (2002) Is there a correlation between chloroplastic and nuclear divergence, or what are the roles of history and selection on genetic diversity in European oaks? Forest Ecol Manag 156(1-3):75– 87
  • Lowe A, Harris S, Ashton P (2008) Ecological genetics: design, analysis, and application. Blackwell, Malden
  • Magri D. Vendramin GG, Comps B, Dupanloup I, Geburek T, Gömöry D, de Beaulieu J-L (2006) A new scenario for the quaternary history of European beech populations: palaeobotanical evidence and genetic consequences. The New Phytologist, 171(1), 199–221
  • Martin L, Jacomet S, Thiebault S (2008) Plant economy during the Neolithic in a mountain context: the case of ‘‘Le Chenet des Pierres’’ in the French Alps (Bozel-Savoie, France). Veg Hist Archaeobot 17:113–122
  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583–590.
  • Orsini L, Vanoverbeke J, Swillen I, Mergeay J, De Meester L (2013) Drivers of population genetic differentiation in the wild: isolation by dispersal limitation, isolation by adaptation and isolation by colonization. Mol Ecol 22(24):5983–5999
  • Petit RJ, Aguinagalde I, de Beaulieu J-L, Bittkau C, Brewer S, Cheddadi R, Vendramin GG (2003) Glacial refugia: hotspots but not melting pots of genetic diversity. Science 300(5625):1563–1565
  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155 (2):945– 959.
  • Raymond M, Rousset F (1995) An exact test for population differentiation. Evolution 49(6):1280
  • Rees M, Condit R, Crawley M, Pacala S, Tilman D (2001) Long-term studies of vegetation dynamics. Science 293(5530):650–655
  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425.
  • Schlüter PM, Harris SA (2006) Analysis of multilocus fingerprinting data sets containing missing data. Mol Ecol Notes 6(2):569–572
  • Schmidt PA, Krause A (1997) Zur Abgrenzung von Herkunftsgebieten bei Baumschulgehölzen für die freie Landschaft. Natur und Landschaft 72:92–95
  • Schütt P, Schuck HJ, Stimm B (eds) (1992) Lexikon der Forstbotanik. Verlag Hüthig, Jehle Rehm, Heidelberg
  • Sokal R and Michener C (1958) A statistical method for University of Kansas Science Bulletin 38: 1409– 1438 relationships".
  • Wright S (1943) Isolation by Distance. Genetics 28(2):114–138

Prunus Spinosa L.’nin Genetik Çeşitliliği ve Yerli Popülasyon Kaynaklarının Kullanımındaki Zorluklar

Yıl 2014, Cilt: 15 Sayı: 2, 149 - 160, 16.01.2015
https://doi.org/10.17474/acuofd.05189

Öz

Almanya’nın farklı bölgelerindeki çakal eriği (Prunus spinosa L.) doğal türünün genetik çeşitliliği, yüksek yapışma sıcaklığında rastgele çoğaltılmış polimorfik DNA (HAT-RAPD) protokolü kullanılarak analiz edilmiştir. Bulgular daha önceki çalışmalarda kaydedilen benzer bölgelerden toplanmış tohum stoklarından elde edilen örneklerin bulgularıyla karşılaştırılmıştır. Genellikle doğal türlerdeki (suş) genetik çeşitlilik önceki çalışmalarda ölçülen değerden (Ho: 0,118-0,113) düşük olduğu (Ho; 0,099-0,116) belirlenmiştir. Dahası genetik farklılaşma doğal türlerde orta düzeyde (Fst; 0,138- 0,184; populasyonlar arası varyasyon oranı % 22,527) ama tohum stoklarından elde edilen örneklerin değerlerinden (Fst; 0,0860,104; populasyonlar arası varyasyon oranı % 7,782) daha yüksek olduğu belirlenmiştir. Bulgular Almanya koruma kanunları ve uygulamaları bakımından tartışılmıştır

Kaynakça

  • Antao T, Lopes A, Lopes RJ, Beja-Pereira A, Luikart, G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9: 323
  • (Bundesministerium BMU für Umwelt,
  • Naturschutz und Reaktorsicherheit) (2012)
  • Leitfaden zur Verwendung gebietseigener
  • Gehölze. Selbstverlag, Berlin
  • BMVEL (Bundesministerium für Verbraucherschutz, Ernährung und Landwirtschaft) (2003) Verwendung einheimischer Gehölze regionaler Herkunft für die freie Landschaft. Ein Beitrag zur Erhaltung und Förderung der biologischen Vielfalt. Selbstverlag, Bonn
  • BnatSchG (Gesetz über Naturschutz und Landschaftspflege) Bundesnaturschutzgesetz vom 29.07.2009, in Kraft getreten am 01.03.2010. BGBl I:2542
  • Convention on biological diversity (1992) Secretariat of the convention on biological diversity. http://www.cbd.int/programmes/outreach/aware ness/publications.shtml. Accessed 18th August 2011
  • Crawford KM, Whitney KD (2010) Population genetic diversity influences colonization success. Mol Ecol 19(6):1253–1263
  • Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297-302
  • Eimert K, Rückert F-E, Schröder M.B. (2011) Genetic diversity within and between seedstock populations of several German autochthonous provenances and conventionally propagated nursery material of blackthorn (Prunus spinosa L.). Plant Syst Evol 298(3):609–618
  • Esquinas-Alcázar J (2005) Science and society: protecting crop genetic diversity for food security: political, ethical and technical challenges. Nat Rev Genet 6(12):946–953
  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14(8):2611–2620
  • Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47–50
  • Fronia R (2009) Prüfung der Identität und Variabilität gebietsfremder Herkünfte von Prunus spinosa L. und Cornus sanguinea L. zur Verwendung in der freien Landschaft. Der Andere Verlag, Tönning, Lübeck, Marburg und Google (2014) Google Earth. Available: http://www.google.com/earth
  • Guitián J, Guitián P, Sánchez JM (1993) Reproductive biology of two Prunus species (Rosaceae) in the Northwest Iberian Peninsula. Plant Syst Evol 185(3-4):153–165
  • Jensen JL, Bohonak AJ, Kelley ST (2005) Isolation by distance, web service. BMC Genet 6(1):13
  • Leinemann L, Bendixen K, Kownatzki D, Hattemer HH, Liepe K, Stenger G (2002) Genetische Untersuchungen an Landschaftsgehölzen im Hinblick auf die Erzeugung und Zertifizierung von Vermehrungsgut. Allgemeine Forst- und Jagdzeitung 173:146-152
  • Leinemann L, Kleinschmit J, Fussi B, Hosius B, Kuchma O, Arenhövel W, Finkeldey R (2014) Genetic composition and differentiation of sloe (Prunus spinosa L.) populations in Germany with respect to the tracing of reproductive plant material. Plant Syst Evol. doi:10.1007/s00606- 014-1027-7
  • Vander Mijnsbrugge K (2013) Genetic and morphological variability among autochthonous Prunus spinosa populations in Flanders (northern part of Belgium): implications for seed sourcing. Plant Ecol Evol 146(2):193–202
  • Mohanty A, Martín JP, Aguinagalde I (2000) Chloroplast DNA diversity within and among populations of the allotetraploid Prunus spinosa L. Theor Appl Genet 100(8):1304–1310
  • Mohanty A, Martín JP, Aguinagalde I (2002) Population genetic analysis of European Prunus spinosa (Rosaceae) using chloroplast DNA markers. Am J Bot 89(8):1223–1228
  • Reynders-Aloisi S, Grellet F (1994) Characterization of the ribosomal DNA units in two related Prunus species (P. Cerasifera and P. Spinosa). Plant Cell Rep 13(11):641–646
  • Hoisington D, Khairallah M, Reeves T, Ribaut J-M, Skovmand B, Taba S, Warburton M (1999) Plant genetic resources: What can they contribute toward increased crop productivity? P Natl Acad Sci USA 96(11):5937–5943
  • Jaccard P (1901) Distribution de la flore alpine dans le bassin des Dranses et dans quelques régions voisines. Bull Soc Vaud Sci Nat 37:241–272
  • Karg S, Markle T (2002) Continuity and changes in plant resources during the Neolithic period in western Switzerland. Veg Hist Archaeobot 11:169–176
  • Karnitsch P (1953) Fundberichte im Jahrbuch der Stadt Linz 1951. Pro Austria Romana 3:26
  • Kimura, M (1968) Evolutionary rate at the molecular level. Nature 217(5129):624–626
  • Kremer A, Kleinschmit J, Cottrell J, Cundall EP, Deans JD, Ducousso A, Stephan BR (2002) Is there a correlation between chloroplastic and nuclear divergence, or what are the roles of history and selection on genetic diversity in European oaks? Forest Ecol Manag 156(1-3):75– 87
  • Lowe A, Harris S, Ashton P (2008) Ecological genetics: design, analysis, and application. Blackwell, Malden
  • Magri D. Vendramin GG, Comps B, Dupanloup I, Geburek T, Gömöry D, de Beaulieu J-L (2006) A new scenario for the quaternary history of European beech populations: palaeobotanical evidence and genetic consequences. The New Phytologist, 171(1), 199–221
  • Martin L, Jacomet S, Thiebault S (2008) Plant economy during the Neolithic in a mountain context: the case of ‘‘Le Chenet des Pierres’’ in the French Alps (Bozel-Savoie, France). Veg Hist Archaeobot 17:113–122
  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583–590.
  • Orsini L, Vanoverbeke J, Swillen I, Mergeay J, De Meester L (2013) Drivers of population genetic differentiation in the wild: isolation by dispersal limitation, isolation by adaptation and isolation by colonization. Mol Ecol 22(24):5983–5999
  • Petit RJ, Aguinagalde I, de Beaulieu J-L, Bittkau C, Brewer S, Cheddadi R, Vendramin GG (2003) Glacial refugia: hotspots but not melting pots of genetic diversity. Science 300(5625):1563–1565
  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155 (2):945– 959.
  • Raymond M, Rousset F (1995) An exact test for population differentiation. Evolution 49(6):1280
  • Rees M, Condit R, Crawley M, Pacala S, Tilman D (2001) Long-term studies of vegetation dynamics. Science 293(5530):650–655
  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425.
  • Schlüter PM, Harris SA (2006) Analysis of multilocus fingerprinting data sets containing missing data. Mol Ecol Notes 6(2):569–572
  • Schmidt PA, Krause A (1997) Zur Abgrenzung von Herkunftsgebieten bei Baumschulgehölzen für die freie Landschaft. Natur und Landschaft 72:92–95
  • Schütt P, Schuck HJ, Stimm B (eds) (1992) Lexikon der Forstbotanik. Verlag Hüthig, Jehle Rehm, Heidelberg
  • Sokal R and Michener C (1958) A statistical method for University of Kansas Science Bulletin 38: 1409– 1438 relationships".
  • Wright S (1943) Isolation by Distance. Genetics 28(2):114–138
Toplam 44 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Klaus Eimert

Yayımlanma Tarihi 16 Ocak 2015
Yayımlandığı Sayı Yıl 2014Cilt: 15 Sayı: 2

Kaynak Göster

APA Eimert, K. (2015). Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 15(2), 149-160. https://doi.org/10.17474/acuofd.05189
AMA Eimert K. Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources. AÇÜOFD. Ocak 2015;15(2):149-160. doi:10.17474/acuofd.05189
Chicago Eimert, Klaus. “Genetic Diversity in Prunus Spinosa L. – Challenges in the Use of Autochthonous Sources”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 15, sy. 2 (Ocak 2015): 149-60. https://doi.org/10.17474/acuofd.05189.
EndNote Eimert K (01 Ocak 2015) Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 15 2 149–160.
IEEE K. Eimert, “Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources”, AÇÜOFD, c. 15, sy. 2, ss. 149–160, 2015, doi: 10.17474/acuofd.05189.
ISNAD Eimert, Klaus. “Genetic Diversity in Prunus Spinosa L. – Challenges in the Use of Autochthonous Sources”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 15/2 (Ocak 2015), 149-160. https://doi.org/10.17474/acuofd.05189.
JAMA Eimert K. Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources. AÇÜOFD. 2015;15:149–160.
MLA Eimert, Klaus. “Genetic Diversity in Prunus Spinosa L. – Challenges in the Use of Autochthonous Sources”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, c. 15, sy. 2, 2015, ss. 149-60, doi:10.17474/acuofd.05189.
Vancouver Eimert K. Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources. AÇÜOFD. 2015;15(2):149-60.
Creative Commons Lisansı
Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Creative Commons Alıntı 4.0 Uluslararası Lisansı ile lisanslanmıştır.