Cilt 16, Sayı 2, Sayfalar 124 - 131 2015-12-30

Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV
Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi

Özlem Saral [1] , Hüseyin Şahin [2] , Mustafa Karaköse [3]

753 733

The aim of the study is to determine phenolic composition and antioxidant activity from Vinca major subsp. hirsuta. Reverse phase high performance liquid chromatography (RP-HPLC-UV) was used to determination phenolic compounds. The antioxidant activity of the plant was investigated in terms of total phenolic content, FRAP, and DPPH radical scavenging assays. The plant is divided into three parts: leaf, flower and stem. According to the applied antioxidant assays, leaf of the plant showed the highest activity. 5 different phenolic compounds in the range of concentrations were determined in the all extracts and parts of Vinca majör subsp. hirsuta by RP-HPLC-UV. Chlorogenic acid was the main ranging from 5344.93 to 112.97 μg phenolic compound/g dry sample, respectively. Gallic acid was found to be lowest in the three parts of the samples. The leaf was observed highest phenolic compounds between the three samples.

Bu çalışma, Vinca major subsp. hirsuta’nın fenolik içeriğinin ve antioksidan aktivitesinin belirlenmesi amacıyla yürütülmüştür. Bitkinin fenolik içeriğinin belirlenmesi için ters fazlı yüksek performanslı sıvı kromatografisi (RP-HPLC-UV) kullanılmıştır. Toplam fenolik madde, FRAP ve DPPH radikal temizleme yöntemleriyle bitkinin antioksidan aktivitesi belirlenmiştir. Bitki yaprak, çiçek ve sap olmak üzere üç kısma ayrılmıştır. Uygulanan antioksidan yöntemlere göre bitkinin yaprak kısmı en yüksek aktivite göstermiştir. RP-HPLC-UV ile Vinca major subsp. hirsuta.’ın tüm kısımlarında ve ekstraktlarında değişik konsantrasyonlardaki 5 farklı fenolik bileşen tespit edilmiştir. Klorojenik asit 5344,93 ila 112,97 µg phenolic bileşen/g kuru örnek birimle ana bileşen olarak belirlenmiştir. Üç örnekte de en düşük fenolik bileşen gallik asit olarak bulunmuştur. Bitkinin üç parçasında en yüksek fenolik bileşen içeriğini ise yaprak göstermiştir.

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Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Özlem Saral
E-posta: kimya061@hotmail.com

Yazar: Hüseyin Şahin
E-posta: huseyinsahin84@gmail.com

Yazar: Mustafa Karaköse
E-posta: mustafakarakose@qwe.com

Bibtex @araştırma makalesi { artvinofd267448, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2015}, volume = {16}, pages = {124 - 131}, doi = {10.17474/acuofd.59141}, title = {Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV}, language = {en}, key = {cite}, author = {Saral, Özlem and Karaköse, Mustafa and Şahin, Hüseyin} } @araştırma makalesi { artvinofd267448, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2015}, volume = {16}, pages = {124 - 131}, doi = {10.17474/acuofd.59141}, title = {Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi}, language = {tr}, key = {cite}, author = {Saral, Özlem and Karaköse, Mustafa and Şahin, Hüseyin} }
APA Saral, Ö , Şahin, H , Karaköse, M . (2015). Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 16 (2), 124-131. DOI: 10.17474/acuofd.59141
MLA Saral, Ö , Şahin, H , Karaköse, M . "Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 16 (2015): 124-131 <http://ofd.artvin.edu.tr/issue/25320/267448>
Chicago Saral, Ö , Şahin, H , Karaköse, M . "Determination of antioxidant activity and phenolic compounds in Vinca major subsp. hirsuta by RP-HPLC-UV". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 16 (2015): 124-131
RIS TY - JOUR T1 - Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi AU - Özlem Saral , Hüseyin Şahin , Mustafa Karaköse Y1 - 2015 PY - 2015 N1 - doi: 10.17474/acuofd.59141 DO - 10.17474/acuofd.59141 T2 - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JF - Journal JO - JOR SP - 124 EP - 131 VL - 16 IS - 2 SN - 2146-1880-2146-698X M3 - doi: 10.17474/acuofd.59141 UR - http://dx.doi.org/10.17474/acuofd.59141 Y2 - 2017 ER -
EndNote %0 Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi %A Özlem Saral , Hüseyin Şahin , Mustafa Karaköse %T Vinca major subsp. hirsuta’nın antioksidan aktivitesinin ve RP-HPLC-UV ile fenolik bileşenlerinin belirlenmesi %D 2015 %J Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi %P 2146-1880-2146-698X %V 16 %N 2 %R doi: 10.17474/acuofd.59141 %U 10.17474/acuofd.59141