Cilt 18, Sayı 1, Sayfalar 55 - 61 2017-04-01

Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)
Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi

Nurcan Albayrak İskender [1] , Serkan Örtücü [2] , Yaşar Aksu [3]

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The fall webworm (Hyphantria cunea) is a polyphagous pest with numerous host plants. In the present study, the bacterial flora of H.cunea was investigated to identify new organisms that can be used as microbial control agent against the pest. Six bacteria were isolated and cultured from H. cunea. Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits) of bacterial isolates were determined. In addition, 16S rRNA gene region was sequenced. As a result of the studies conducted, bacterial isolates were identified as Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) and Microbacterium oxydans (Abk8). All bacterial isolates were tested for 12 days against third-fourth instar larvae of H. cunea. The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p<0.05). These results indicate that L. sphaericus (Abk1) can be taken into account in the microbial pest control of H. cunea. In the future, further studies will be conducted by using pathogenicity enrichment strategies of L. sphaericus (Abk1) (ex. combining with other entomopathogens or insecticides) in order to increase the effectiveness on H. cunea.


Amerikan Beyaz Kelebeği, Hyphantria cunea (Drury) sayısız konukçu bitkisi olan polifag bir zararlıdır.  Bu çalışmada, H. cunea’nın bakteriyal florası zararlıya karşı mikrobiyal kontrol ajanı olabilecek yeni organizmaları identifiye etmek için araştırıldı. H. cunea’dan altı bakteri izole edilerek saflaştırıldı. Bakteriyal izolatların morfolojik, biyokimyasal, diğer fenotipik özellikleri (API 20E, API 50 CH, API Staph ve API Coryne) belirlendi. İlave olarak, 16S rRNA gen sekans analizi de yapıldı. Yapılan çalışmalar sonucunda izolatlar; Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) ve Microbacterium oxydans (Abk8) olarak tanımlandı. Bütün bakteriler H. cunea’nın üçüncü-dördüncü gömlek larvalarına karşı oniki gün boyunca test edildi. En yüksek insektisidal aktivite %30 ile L. sphaericus (Abk1)’dan elde edildi (p<0.05). Bu sonuçlar, L. sphaericus (Abk1)’un, H. cunea’nın mikrobiyal mücadelesinde dikkate alınabileceğini göstermiştir. Gelecekte, L. sphaericus (Abk1)’un patojenite zenginleştirme stratejileri (diğer entomopatojelerle veya insektisitlerle kombine edilmesi gibi) kullanılarak H. cunea üzerindeki etkinliğinin artırılması yönünde çalışmalar yapılacaktır.

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Konular Yaşam Bilimleri
Yayımlanma Tarihi Nisan
Dergi Bölümü Araştırma Makalesi
Yazarlar

Yazar: Nurcan Albayrak İskender
E-posta: nurcaniskender25@hotmail.com
Ülke: Turkey


Yazar: Serkan Örtücü
E-posta: serkanortucu@gmail.com

Yazar: Yaşar Aksu
E-posta: y_aksu3@hotmail.com

Bibtex @araştırma makalesi { artvinofd274071, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2017}, volume = {18}, pages = {55 - 61}, doi = {10.17474/artvinofd.274071}, title = {Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)}, language = {en}, key = {cite}, author = {Aksu, Yaşar and Örtücü, Serkan and Albayrak İskender, Nurcan} } @araştırma makalesi { artvinofd274071, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, address = {Artvin Çoruh Üniversitesi}, year = {2017}, volume = {18}, pages = {55 - 61}, doi = {10.17474/artvinofd.274071}, title = {Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi}, language = {tr}, key = {cite}, author = {Aksu, Yaşar and Örtücü, Serkan and Albayrak İskender, Nurcan} }
APA Albayrak İskender, N , Örtücü, S , Aksu, Y . (2017). Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 18 (1), 55-61. DOI: 10.17474/artvinofd.274071
MLA Albayrak İskender, N , Örtücü, S , Aksu, Y . "Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 18 (2017): 55-61 <http://ofd.artvin.edu.tr/issue/25618/274071>
Chicago Albayrak İskender, N , Örtücü, S , Aksu, Y . "Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 18 (2017): 55-61
RIS TY - JOUR T1 - Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi AU - Nurcan Albayrak İskender , Serkan Örtücü , Yaşar Aksu Y1 - 2017 PY - 2017 N1 - doi: 10.17474/artvinofd.274071 DO - 10.17474/artvinofd.274071 T2 - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JF - Journal JO - JOR SP - 55 EP - 61 VL - 18 IS - 1 SN - 2146-1880-2146-698X M3 - doi: 10.17474/artvinofd.274071 UR - http://dx.doi.org/10.17474/artvinofd.274071 Y2 - 2017 ER -
EndNote %0 Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi %A Nurcan Albayrak İskender , Serkan Örtücü , Yaşar Aksu %T Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi %D 2017 %J Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi %P 2146-1880-2146-698X %V 18 %N 1 %R doi: 10.17474/artvinofd.274071 %U 10.17474/artvinofd.274071