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Türkiye'de Hyphantria cunea larvalarından izole edilen iki yeni entomopatojenik Beauveria bassiana suşunun karakterizasyonu ve biyolojik aktivitesi

Yıl 2022, Cilt 23, Sayı 2, 209 - 216, 28.10.2022
https://doi.org/10.17474/artvinofd.1181151

Öz

Bu çalışmada, Hyphantria cunea (Amerikan beyaz kelebeği) larvalarından izole edilen iki fungus suşunun (HC-Z1 ve HC-Z2), H. cunea larvalarına karşı biyokontrol etmeni olarak potansiyelleri araştırıldı. Morfolojik ve moleküler (ITS1-5.8S-ITS2 bölgesinin dizileri) karakterizasyonlarına göre suşlar Beauveria bassiana (HC-Z1: OP415530 ve HC-Z2: OP415531) olarak tanımlandı. İki fungus suşunun biyolojik aktivitelerini belirlemek için H. cunea larvalarının 3. evresinde konsantrasyon yanıt testi (1 × 104-8 konidia/ml) yapıldı. Ayrıca, iki B. bassiana suşu, 1 × 107 konidia/ml konsantrasyonu H. cunea larvalarının beş evresi (1-5.) üzerinde test edildi. Her iki suş da H. cunea'nın birinci evre larvalarına karşı 7 gün içinde %96’nın üzerinde ölüm oranı oluşturdu. Üçüncü evre H. cunea larvalarına karşı HC-Z1 ve HC-Z2 suşlarının LC50 değerleri sırasıyla 0.6 × 104 ve 1.2 × 104 konidia/ml olarak hesaplandı. LT50 değerleri ise HC-Z1 ve HC-Z2 suşları için sırasıyla 2.82 ve 3.44 gün olarak belirlenmiştir. Sonuç olarak, HC-Z1 ve HC-Z2 suşlarının potansiyel olarak H. cunea'ya karşı biyolojik kontrol etmeni olarak kullanılabileceği sonucuna varılabilir.

Kaynakça

  • Ajamhassani M, Sendi JJ, Zibaee A, Askary H, Farsi MJ (2013) Immunoliogical responses of Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) to entomopathogenic fungi, Beauveria bassiana (Bals.-Criy) and Isaria farinosae (Holmsk.) Fr. J Plant Prot Res 53:2
  • Aker O, Kushiyev R (2016) Pathogenicity of Metarhizium anisopliae on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. Int J Zool Stud 1(6):29-32
  • Aker O, Tuncer C (2016a) Efficacy of Metarhizium anisopliae and some entomopathogenic fungi on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). J Entomol Zool Stud 4(5):171-176
  • Aker O, Tuncer C (2016b) Pathogenicity of Beauveria bassiana on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. Int J Entomol Res 1(6):16-20
  • Ansari MA, Brownbridge M, Shah FA, Butt TM (2008) Efficacy of entomopathogenic fungi against soil-dwelling life stages of western flower thrips, Frankliniella occidentalis, in plant-growing media. Entomol Exp Appl 127:80-87
  • Bayramoğlu Z, Nalçacıoğlu R, Demirbağ Z, Demir İ (2018) Characterization of a Betabaculovirus from the fall webworm, Hyphantria cunea Drury. (Lepidoptera: Erebidae), in Turkey. Biocontrol Sci Technol 28(12):1178-1190
  • Benson DA, Karsch-Mizrachi I, Clark K, Lipman DJ, Ostell J, Sayers EW (2012) GenBank. Nucleic Acids Res 40:48-53
  • Chen WH, Man L, Huang ZX, Yang GM, Han YF, Liang JD, Liang ZQ (2018) Beauveria majiangensis, a new entomopathogenic fungus from Guizhou, China. Phytotaxa 333:243-250
  • Dembilio Ó, Quesada-Moraga E, Santiago-Álvarez C, Jacas JA (2010) Potential of an indigenous strain of the entomopathogenic fungus Beauveria bassiana as a biological control agent against the Red Palm Weevil Rhynchophorus ferrugineus. J Invertebr Pathol 104(3):214-221
  • Edosa TT, Jo YH, Keshavarz M, Anh YS, Noh MY, Han YS (2019) Current status of the management of fall webworm, Hyphantria cunea: Towards the integrated pest management development. J Appl Entomol 143:1-10
  • Finney DJ (1971) Probit Analysis. Cambridge University Press, Cambridge, UK
  • Greenfield M, Gomez-Jimenez MI, Ortiz V, Vega FE, Kramer M, Parsa S (2016) Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biol Control 95:40-48
  • Gurulingappa P, Gee PM, Sword GA (2011) In Vitro and In Planta Compatibility of Insecticides and the Endophytic Entomopathogen, Lecanicillium lecanii. Mycopathologia 172:161-168
  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95-98
  • Humber RA (1997) Identification of entomopathogenic fungi. In: Lacey LA (ed) Manual of Techniques in Insect Pathology, Academic Press, London, UK, pp 151-187
  • Humber RA (2012) Identification of entomopathogenic fungi. In: Lacey LA (ed) In Manual of Techniques in Invertebrate Pathology. Elsevier, 32 Jamestown Road, London, UK, pp 151-187
  • İskender NA, Örtücü S, Aksu Y (2012) Pathogenicity of three isolates of the entomopathogenic Fungi Beauveria bassiana to control Hyphantria cunea Drury Lepidoptera: Arctiidae Larvae. Manas Fen Bilimleri Dergisi 2(13):15-21
  • Ito Y, Miyashita K (1968) Biology of Hyphantria cunea Drury (Lepidoptera: Arctiidae) in Japan. V. Preliminary life tables and mortality data in urban areas. Res Popul Ecol 10:177-209
  • Jialin L, Jingyun C, Ping C (2013) Research Progress of Occurrence and Comprehensive Control of Fall Webworm [Hyphantria cunea (Drury)]. Plant Dis Pests 4(4):32-35
  • Karabörklü S, Altın N, Bahadır A (2022) Efficiency of Native Beauveria bassiana and Metarhizium anisopliae Isolates against the Early Instar Larvae of fall webworm, Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebiidae). Ziraat Mühendisliği 373:4-9
  • Karabörklü S, Altın N, Yıldırım İ, Öztemiz S, Sadıç E, Aydın Ö (2020) Insecticidal activity of some Turkish strains of entomopathogenic fungi against the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) under the laboratory conditions. Türkiye Biyol Mücadele Derg 11(1):119-128
  • Kumar S, Stecher A, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis Across Computing Platforms. Mol Biol Evol 35(6):1547-1549
  • Meyling NV, Eilenberg J (2007) Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control. Biol Control 43:145-155
  • Qin F-q, Yan G-z, He W, Wang J-l, Tao W-q (2012) Elementary Study on Control of Hyphantria cunea by Beauveria bassiana and Paecilomyces farinosus. J Shanxi Agric Sci 17(02):140-145
  • Rehner SA, Buckley EP (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84-98.
  • Saruhan İ, Toksöz Ş, Erper İ (2017) Evaluation of some entomopathogenic fungi against the fall webworm (Hyphantria cunea Durry, Lepidoptera: Arctidae). Selcuk J Agr Food Sci 31(2):76-81
  • Saruhan, I., Akca, I., and Kushiyev, R. (2014). Toxicity of some biopesticides to the fall webworm, Hyphantria cunea Durry (Lepidoptera: Arctidae). Egypt. J. Biol. Pest. Control 24, 255–257.
  • Schowalter TD, Ring DR (2017) Biology and management of the fall webworm, Hyphantria cunea (Lepidoptera: Erebidae). J Integr Pest Manag 8(1):7
  • Shah PA, Pell JK (2003) Entomopathogenic fungi as biological control agents. Appl Microbiol Biotechnol 61(5-6):413-423
  • Sullivan GT (2011) Determination of Biological Control Agents of Overwintering Hyphantria cunea Pupae and Efficacy of Entomopathogenic Fungi. Master thesis Süleyman Demirel University Graduate School of Applied and Natural Sciences, Isparta
  • Sullivan GT, Özman-Sullivan S, Karaca I, Karaca G (2011) Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’nın kışlayan pupalarından elde edilen fungusların entomopatojen etkilerinin belirlenmesi. In: Türkiye IV. Bitki Koruma Kongresi Bildirileri. Kahramanmaraş, Turkey, pp 137
  • Takatsuka J (2007) Characterization of Beauveria bassiana isolates from Japan using inter-simple-sequence-repeat-anchored-polymerase chain reaction (ISSR-PCR) amplification. Appl Entomol Zool 42:563-571
  • Tamura K, Stecher G, Peterson D, Filipski A (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725-2729
  • Tuncer C, Kansu IA (1994) Konukçu Bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)’ya etkileri üzerinde araştırmalar. Turk Entomol Derg 18(4):209-222
  • Wang W, Zhou L, Dong G, Chen F (2020) Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). BioControl 1-11
  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315-322
  • Xiong Q, Xiea Y, Zhua Y, Xuea J, Li J, Fan R (2013) Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae). Micron 44:303-311
  • Yang ZQ, Wang XY, Wei JR, Qu HR, Qiao XR (2008) Survey of the native insect natural enemies of Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) in China. Bull Entomol Res 98:293-302
  • Yang ZQ, Wei JR, Wang XY (2006) Mass rearing and augmentative releases of the native parasitoid Chouioia cunea for biological control of the introduced fall webworm Hyphantria cunea in China. Biocontrol 51:401-418
  • Zare R, Gams W (2001) A revision of Verticillium sect. Prostata IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia 73:1-50
  • Zhang L, Kang K, Liu Y, Zhang T, Sun L, Zhan C, Huang C, Jiang L, Ye K, Ding D (2016) Evaluation of Beauveria bassiana isolates as potential agents for control of Hyphantria cunea (Lepidoptera: Arctiidae). Acta Entomol Sin 59(1):111-118
  • Zibaee I, Bandani AR, Sendi JJ (2013) Pathogenicity of Beauveria bassiana to fall webworm (Hyphantria cunea) (Lepidoptera: Arctiidae) on different host plants. Plant Prot Sci 49(4):169-176
  • Zimmermann G (2008) The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont Sci Technol 18:865-901

Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey

Yıl 2022, Cilt 23, Sayı 2, 209 - 216, 28.10.2022
https://doi.org/10.17474/artvinofd.1181151

Öz

In this study, two fungal strains (HC-Z1 and HC-Z2) from Hyphantria cunea (fall webworm) larvae were evaluated for their potential as biocontrol agents against the H. cunea larvae. Based on morphological and molecular (ITS1-5.8S-ITS2 region) characterization, the strains were identified as Beauveria bassiana (HC-Z1: OP415530 and HC-Z2: OP415531). To determine the biological activities of the two fungal strains, a concentration-response assay (1 × 104-8 conidia/ml) was performed against third stage H. cunea larvae. In addition, two B. bassiana strains were tested on five (1-5) larval stages at 1 × 107 conidia/ml concentration. Both isolates produced mortalities over 96% within 7 days for the first larval stage of H. cunea. The LC50 and LT50 of HC-Z1 and HC-Z2 strains against third instar H. cunea larvae were calculated as 0.6 × 104 and 1.2 × 104 conidia/ml, respectively. LT50 values were obtained in 2.82 and 3.44 days for HC-Z1 and HC-Z2 isolates, respectively. As a result, it can be concluded that HC-Z1 and HC-Z2 strains can potentially be used as biological control agents against H. cunea.

Kaynakça

  • Ajamhassani M, Sendi JJ, Zibaee A, Askary H, Farsi MJ (2013) Immunoliogical responses of Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) to entomopathogenic fungi, Beauveria bassiana (Bals.-Criy) and Isaria farinosae (Holmsk.) Fr. J Plant Prot Res 53:2
  • Aker O, Kushiyev R (2016) Pathogenicity of Metarhizium anisopliae on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. Int J Zool Stud 1(6):29-32
  • Aker O, Tuncer C (2016a) Efficacy of Metarhizium anisopliae and some entomopathogenic fungi on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). J Entomol Zool Stud 4(5):171-176
  • Aker O, Tuncer C (2016b) Pathogenicity of Beauveria bassiana on larvae of fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) at different temperatures. Int J Entomol Res 1(6):16-20
  • Ansari MA, Brownbridge M, Shah FA, Butt TM (2008) Efficacy of entomopathogenic fungi against soil-dwelling life stages of western flower thrips, Frankliniella occidentalis, in plant-growing media. Entomol Exp Appl 127:80-87
  • Bayramoğlu Z, Nalçacıoğlu R, Demirbağ Z, Demir İ (2018) Characterization of a Betabaculovirus from the fall webworm, Hyphantria cunea Drury. (Lepidoptera: Erebidae), in Turkey. Biocontrol Sci Technol 28(12):1178-1190
  • Benson DA, Karsch-Mizrachi I, Clark K, Lipman DJ, Ostell J, Sayers EW (2012) GenBank. Nucleic Acids Res 40:48-53
  • Chen WH, Man L, Huang ZX, Yang GM, Han YF, Liang JD, Liang ZQ (2018) Beauveria majiangensis, a new entomopathogenic fungus from Guizhou, China. Phytotaxa 333:243-250
  • Dembilio Ó, Quesada-Moraga E, Santiago-Álvarez C, Jacas JA (2010) Potential of an indigenous strain of the entomopathogenic fungus Beauveria bassiana as a biological control agent against the Red Palm Weevil Rhynchophorus ferrugineus. J Invertebr Pathol 104(3):214-221
  • Edosa TT, Jo YH, Keshavarz M, Anh YS, Noh MY, Han YS (2019) Current status of the management of fall webworm, Hyphantria cunea: Towards the integrated pest management development. J Appl Entomol 143:1-10
  • Finney DJ (1971) Probit Analysis. Cambridge University Press, Cambridge, UK
  • Greenfield M, Gomez-Jimenez MI, Ortiz V, Vega FE, Kramer M, Parsa S (2016) Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biol Control 95:40-48
  • Gurulingappa P, Gee PM, Sword GA (2011) In Vitro and In Planta Compatibility of Insecticides and the Endophytic Entomopathogen, Lecanicillium lecanii. Mycopathologia 172:161-168
  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95-98
  • Humber RA (1997) Identification of entomopathogenic fungi. In: Lacey LA (ed) Manual of Techniques in Insect Pathology, Academic Press, London, UK, pp 151-187
  • Humber RA (2012) Identification of entomopathogenic fungi. In: Lacey LA (ed) In Manual of Techniques in Invertebrate Pathology. Elsevier, 32 Jamestown Road, London, UK, pp 151-187
  • İskender NA, Örtücü S, Aksu Y (2012) Pathogenicity of three isolates of the entomopathogenic Fungi Beauveria bassiana to control Hyphantria cunea Drury Lepidoptera: Arctiidae Larvae. Manas Fen Bilimleri Dergisi 2(13):15-21
  • Ito Y, Miyashita K (1968) Biology of Hyphantria cunea Drury (Lepidoptera: Arctiidae) in Japan. V. Preliminary life tables and mortality data in urban areas. Res Popul Ecol 10:177-209
  • Jialin L, Jingyun C, Ping C (2013) Research Progress of Occurrence and Comprehensive Control of Fall Webworm [Hyphantria cunea (Drury)]. Plant Dis Pests 4(4):32-35
  • Karabörklü S, Altın N, Bahadır A (2022) Efficiency of Native Beauveria bassiana and Metarhizium anisopliae Isolates against the Early Instar Larvae of fall webworm, Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebiidae). Ziraat Mühendisliği 373:4-9
  • Karabörklü S, Altın N, Yıldırım İ, Öztemiz S, Sadıç E, Aydın Ö (2020) Insecticidal activity of some Turkish strains of entomopathogenic fungi against the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) under the laboratory conditions. Türkiye Biyol Mücadele Derg 11(1):119-128
  • Kumar S, Stecher A, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis Across Computing Platforms. Mol Biol Evol 35(6):1547-1549
  • Meyling NV, Eilenberg J (2007) Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control. Biol Control 43:145-155
  • Qin F-q, Yan G-z, He W, Wang J-l, Tao W-q (2012) Elementary Study on Control of Hyphantria cunea by Beauveria bassiana and Paecilomyces farinosus. J Shanxi Agric Sci 17(02):140-145
  • Rehner SA, Buckley EP (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97:84-98.
  • Saruhan İ, Toksöz Ş, Erper İ (2017) Evaluation of some entomopathogenic fungi against the fall webworm (Hyphantria cunea Durry, Lepidoptera: Arctidae). Selcuk J Agr Food Sci 31(2):76-81
  • Saruhan, I., Akca, I., and Kushiyev, R. (2014). Toxicity of some biopesticides to the fall webworm, Hyphantria cunea Durry (Lepidoptera: Arctidae). Egypt. J. Biol. Pest. Control 24, 255–257.
  • Schowalter TD, Ring DR (2017) Biology and management of the fall webworm, Hyphantria cunea (Lepidoptera: Erebidae). J Integr Pest Manag 8(1):7
  • Shah PA, Pell JK (2003) Entomopathogenic fungi as biological control agents. Appl Microbiol Biotechnol 61(5-6):413-423
  • Sullivan GT (2011) Determination of Biological Control Agents of Overwintering Hyphantria cunea Pupae and Efficacy of Entomopathogenic Fungi. Master thesis Süleyman Demirel University Graduate School of Applied and Natural Sciences, Isparta
  • Sullivan GT, Özman-Sullivan S, Karaca I, Karaca G (2011) Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’nın kışlayan pupalarından elde edilen fungusların entomopatojen etkilerinin belirlenmesi. In: Türkiye IV. Bitki Koruma Kongresi Bildirileri. Kahramanmaraş, Turkey, pp 137
  • Takatsuka J (2007) Characterization of Beauveria bassiana isolates from Japan using inter-simple-sequence-repeat-anchored-polymerase chain reaction (ISSR-PCR) amplification. Appl Entomol Zool 42:563-571
  • Tamura K, Stecher G, Peterson D, Filipski A (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725-2729
  • Tuncer C, Kansu IA (1994) Konukçu Bitkilerin Hyphantria cunea (Drury) (Lepidoptera, Arctiidae)’ya etkileri üzerinde araştırmalar. Turk Entomol Derg 18(4):209-222
  • Wang W, Zhou L, Dong G, Chen F (2020) Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). BioControl 1-11
  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315-322
  • Xiong Q, Xiea Y, Zhua Y, Xuea J, Li J, Fan R (2013) Morphological and ultrastructural characterization of Carposina sasakii larvae (Lepidoptera: Carposinidae) infected by Beauveria bassiana (Ascomycota: Hypocreales: Clavicipitaceae). Micron 44:303-311
  • Yang ZQ, Wang XY, Wei JR, Qu HR, Qiao XR (2008) Survey of the native insect natural enemies of Hyphantria cunea (Drury) (Lepidoptera: Arctiidae) in China. Bull Entomol Res 98:293-302
  • Yang ZQ, Wei JR, Wang XY (2006) Mass rearing and augmentative releases of the native parasitoid Chouioia cunea for biological control of the introduced fall webworm Hyphantria cunea in China. Biocontrol 51:401-418
  • Zare R, Gams W (2001) A revision of Verticillium sect. Prostata IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia 73:1-50
  • Zhang L, Kang K, Liu Y, Zhang T, Sun L, Zhan C, Huang C, Jiang L, Ye K, Ding D (2016) Evaluation of Beauveria bassiana isolates as potential agents for control of Hyphantria cunea (Lepidoptera: Arctiidae). Acta Entomol Sin 59(1):111-118
  • Zibaee I, Bandani AR, Sendi JJ (2013) Pathogenicity of Beauveria bassiana to fall webworm (Hyphantria cunea) (Lepidoptera: Arctiidae) on different host plants. Plant Prot Sci 49(4):169-176
  • Zimmermann G (2008) The entomopathogenic fungi Isaria farinosa (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocont Sci Technol 18:865-901

Ayrıntılar

Birincil Dil İngilizce
Konular Biyoloji, Ziraat
Bölüm Araştırma Makalesi
Yazarlar

Zeynep BAYRAMOĞLU> (Sorumlu Yazar)
RECEP TAYYİP ERDOĞAN ÜNİVERSİTESİ, PAZAR MESLEK YÜKSEKOKULU
0000-0001-6994-1106
Afghanistan

Yayımlanma Tarihi 28 Ekim 2022
Yayınlandığı Sayı Yıl 2022, Cilt 23, Sayı 2

Kaynak Göster

Bibtex @araştırma makalesi { artvinofd1181151, journal = {Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi}, issn = {2146-1880}, eissn = {2146-698X}, address = {}, publisher = {Artvin Çoruh Üniversitesi}, year = {2022}, volume = {23}, number = {2}, pages = {209 - 216}, doi = {10.17474/artvinofd.1181151}, title = {Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey}, key = {cite}, author = {Bayramoğlu, Zeynep} }
APA Bayramoğlu, Z. (2022). Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey . Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi , 23 (2) , 209-216 . DOI: 10.17474/artvinofd.1181151
MLA Bayramoğlu, Z. "Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey" . Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 23 (2022 ): 209-216 <https://ofd.artvin.edu.tr/tr/pub/issue/73139/1181151>
Chicago Bayramoğlu, Z. "Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 23 (2022 ): 209-216
RIS TY - JOUR T1 - Türkiye'de Hyphantria cunea larvalarından izole edilen iki yeni entomopatojenik Beauveria bassiana suşunun karakterizasyonu ve biyolojik aktivitesi AU - ZeynepBayramoğlu Y1 - 2022 PY - 2022 N1 - doi: 10.17474/artvinofd.1181151 DO - 10.17474/artvinofd.1181151 T2 - Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi JF - Journal JO - JOR SP - 209 EP - 216 VL - 23 IS - 2 SN - 2146-1880-2146-698X M3 - doi: 10.17474/artvinofd.1181151 UR - https://doi.org/10.17474/artvinofd.1181151 Y2 - 2022 ER -
EndNote %0 Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey %A Zeynep Bayramoğlu %T Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey %D 2022 %J Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi %P 2146-1880-2146-698X %V 23 %N 2 %R doi: 10.17474/artvinofd.1181151 %U 10.17474/artvinofd.1181151
ISNAD Bayramoğlu, Zeynep . "Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey". Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 23 / 2 (Ekim 2022): 209-216 . https://doi.org/10.17474/artvinofd.1181151
AMA Bayramoğlu Z. Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey. AÇÜOFD. 2022; 23(2): 209-216.
Vancouver Bayramoğlu Z. Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi. 2022; 23(2): 209-216.
IEEE Z. Bayramoğlu , "Characterization and biological activity of two new entomopathogenic Beauveria bassiana strains isolated from Hyphantria cunea larvae in Turkey", Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, c. 23, sayı. 2, ss. 209-216, Eki. 2022, doi:10.17474/artvinofd.1181151
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