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Determination of antimicrobial effect of Lactarius controversus (Pers.) Pers

Year 2024, Volume: 25 Issue: 1, 1 - 5, 15.05.2024
https://doi.org/10.17474/artvinofd.1335292

Abstract

Lactarius controversus (Pers.) Pers., known as willow mushroom, is an edible species by the public. Although many people in the world do not prefer to consume it due to its acrid taste, it is widely consumed in our country. In this study, the antimicrobial effects of methanol and ethanol extracts of L. controversus against some microorganisms (Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa DMS50071, Bacillus megaterium DSM32, Staphylococcus aureus COWAN1, Candida albicans FMC17, Candida globrata ATCC 66032 and Trichophyton sp.) were investigated. According to the disk diffusion method, it has been determined that methanol and ethanol extracts of L. controversus have an antimicrobial effect (8.3-25.3mm) against microorganisms used. According to the microdilution method, it was determined that the minimum inhibitory concentration value of methanol and ethanol extracts of the same species was between 31.25-250 µg/mL. As a result of the study, it may be recommended to use L. controversus as an antimicrobial agent against bacteria, yeast, and dermatophytes.

References

  • Agarwal A, Gupta V, Yadav AN, Sain D, Rahi RK, Bera SP, Neelam D (2023) Aspects of mushrooms and theır extracts as natural antımıcrobıal agents: microbiology. Journal of Microbiology, Biotechnology and Food Sciences, 12(6):e9191-e9191.
  • Altuner EM, Akata I (2010) Antimicrobial activity of some macrofungi extracts. Sakarya Üniversitesi Fen Bilimleri Dergisi, 14(1):45-49.
  • Avcı E, Avci GA (2019) Antimicrobial and antioxidant activities of medicinally important Lactarius deliciosus. International Journal of Medical Science and Dental Research, 2(6):49-55.
  • Babu DR, Pandey M, Rao GN (2014) Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants. Journal of food science and technology, 51:3317-3324.
  • Collins CM, Lyne PM (2004) Microbiological methods. In: Collins CH, Lyne PM, Grange JM, Flkinham III JO (ed) Mycological methods, 8 ed. London, p.140.
  • Dulger B, Yilmaz F, Gucin F (2002) Antimicrobial activity of some Lactarius species. Pharmaceutical Biology, 40:304-306.
  • Erbiai EH, Bouchra B, da Silva LP, Lamrani Z, Pinto E, da Silva JCE, Maouni A (2021) Chemical composition and antioxidant and antimicrobial activities of Lactarius sanguifluus, a wild edible mushroom from northern Morocco. Euro-Mediterranean Journal for Environmental Integration, 6:1-12.
  • EUCAST (European Committee on Antimicrobial Susceptibility Testing) (2022) EUCAST reading guide for broth microdilution. Version 4.0.
  • Heilmann-Clausen J, Verbeken A, Vesterholt J (2000) The Genus Lactarius. In: Petersen LJH, Elborne SA (ed) Fungi of Northern Europe 2, Denmark.
  • Khan NH, Kamal MSA, Rahman M (1988) Antibacterial activity of Euphorbia thymifolia Linn. Indian Journal of Medicinal Research, 87:395–397.
  • Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Sokoviç M, Ćirić A (2023) A Comparative study of Lactarius mushrooms: chemical characterization, antibacterial, antibiofilm, antioxidant and cytotoxic activity. Journal of Fungi, 9(1):70.
  • Mayirnao HS, Gupta S, Thokchom SD, Sharma K, Mehmood T, Kaur S, Sharma YP, Kapoor R (2023) Nutritional assessment of Lactarius drassinus and L. controversus from the cold desert region of the Northwest Himalayas for their potential as food supplements. Journal of Fungi, 9(7):763.
  • Moussa AY, Fayez S, Xiao H, Xu B (2022) New insights into antimicrobial and antibiofilm effects of edible mushrooms. Food Research International, 111982.
  • Niu G, Li W (2019) Next-generation drug discovery to combat antimicrobial resistance. Trends in Biochemical Sciences, 44(11):961-972.
  • Novaković AR, Karaman MA, Milovanović IL, Belović MM, Rašeta MJ, Radusin TI, Ilić NM (2016) Edible mycorrhizal species Lactarius controversus Pers. 1800 as a source of antioxidant and cytotoxic agents. Hemijska Industrija, 70(2):113-122.
  • Özen T, Darcan C, Kaygusuz Ö, Türkekul İ (2016) The Chemical content, antioxidant and antimicrobial assays of Lactarius controversus and Lactarius musteus: two edible wild mushrooms from Giresun province of Turkey. Annals of Food Processing and Preservation, 1(1):1001.
  • Pancu DF, Scurtu A, Macasoi IG, Marti D, Mioc M, Soica C, Coricovac D, Horhat D, Poenaru M, Dehelean C (2021) Antibiotics: conventional therapy and natural compounds with antibacterial activity a pharmaco-toxicological screening. Antibiotics, 10(4):401.
  • Pekşen A, Kibar B, Yakupoğlu G (2007) Yenilebilir bazı Lactarius türlerinin morfolojik özelliklerinin, protein ve mineral içeriklerinin belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 22(3):301-305.
  • Stanković M, Mitić V, Stankov Jovanović V, Dimitrijević M, Nikolić J, Stojanović G (2022) Selected fungi of the genus Lactarius screening of antioxidant capacity, antimicrobial activity, and genotoxicity. Journal of Toxicology and Environmental Health, Part A, 85(17):699-714.
  • Suleiman WB, Shehata RM, Younis AM (2022) In vitro assessment of multipotential therapeutic importance of Hericium erinaceus mushroom extracts using different solvents. Bioresources and Bioprocessing, 9(1):1-13.

Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi

Year 2024, Volume: 25 Issue: 1, 1 - 5, 15.05.2024
https://doi.org/10.17474/artvinofd.1335292

Abstract

Söğüt mantarı olarak bilinen Lactarius controversus (Pers.) Pers., halk tarafından yenilebilir bir türdür. Dünyadaki birçok insan buruk tadı nedeniyle tüketmeyi tercih etmese de ülkemizde yaygın olarak tüketilmektedir. Bu çalışmada, L. controversus’un metanol ve etanol ekstraktlarının bazı mikroorganizmalara (Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC700603, Pseudomonas aeruginosa DMS50071, Bacillus megaterium DSM32, Staphylococcus aureus COWAN1, Candida albicans FMC17, Candida glabrata ATCC 66032 ve Trichophyton sp.) karşı antimikrobiyal etkileri araştırılmıştır. Disk difüzyon yöntemine göre L. controversus'un metanol ve etanol ekstraktlarının kullanılan mikroorganizmalara karşı antimikrobiyal (8.3-25.3 mm) etkiye sahip olduğu belirlenmiştir. Mikrodilüsyon yöntemine göre aynı türe ait metanol ve etanol ekstraktlarının minimum inhibitör konsantrasyon değerinin 31.25-250 µg/mL arasında olduğu tespit edilmiştir. Çalışma sonucunda L. controversus'un bakteri, maya ve dermatofitlere karşı antimikrobiyal ajan olarak kullanılabileceği belirlenmiştir.

References

  • Agarwal A, Gupta V, Yadav AN, Sain D, Rahi RK, Bera SP, Neelam D (2023) Aspects of mushrooms and theır extracts as natural antımıcrobıal agents: microbiology. Journal of Microbiology, Biotechnology and Food Sciences, 12(6):e9191-e9191.
  • Altuner EM, Akata I (2010) Antimicrobial activity of some macrofungi extracts. Sakarya Üniversitesi Fen Bilimleri Dergisi, 14(1):45-49.
  • Avcı E, Avci GA (2019) Antimicrobial and antioxidant activities of medicinally important Lactarius deliciosus. International Journal of Medical Science and Dental Research, 2(6):49-55.
  • Babu DR, Pandey M, Rao GN (2014) Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants. Journal of food science and technology, 51:3317-3324.
  • Collins CM, Lyne PM (2004) Microbiological methods. In: Collins CH, Lyne PM, Grange JM, Flkinham III JO (ed) Mycological methods, 8 ed. London, p.140.
  • Dulger B, Yilmaz F, Gucin F (2002) Antimicrobial activity of some Lactarius species. Pharmaceutical Biology, 40:304-306.
  • Erbiai EH, Bouchra B, da Silva LP, Lamrani Z, Pinto E, da Silva JCE, Maouni A (2021) Chemical composition and antioxidant and antimicrobial activities of Lactarius sanguifluus, a wild edible mushroom from northern Morocco. Euro-Mediterranean Journal for Environmental Integration, 6:1-12.
  • EUCAST (European Committee on Antimicrobial Susceptibility Testing) (2022) EUCAST reading guide for broth microdilution. Version 4.0.
  • Heilmann-Clausen J, Verbeken A, Vesterholt J (2000) The Genus Lactarius. In: Petersen LJH, Elborne SA (ed) Fungi of Northern Europe 2, Denmark.
  • Khan NH, Kamal MSA, Rahman M (1988) Antibacterial activity of Euphorbia thymifolia Linn. Indian Journal of Medicinal Research, 87:395–397.
  • Kostić M, Ivanov M, Fernandes Â, Calhelha RC, Glamočlija J, Barros L, Sokoviç M, Ćirić A (2023) A Comparative study of Lactarius mushrooms: chemical characterization, antibacterial, antibiofilm, antioxidant and cytotoxic activity. Journal of Fungi, 9(1):70.
  • Mayirnao HS, Gupta S, Thokchom SD, Sharma K, Mehmood T, Kaur S, Sharma YP, Kapoor R (2023) Nutritional assessment of Lactarius drassinus and L. controversus from the cold desert region of the Northwest Himalayas for their potential as food supplements. Journal of Fungi, 9(7):763.
  • Moussa AY, Fayez S, Xiao H, Xu B (2022) New insights into antimicrobial and antibiofilm effects of edible mushrooms. Food Research International, 111982.
  • Niu G, Li W (2019) Next-generation drug discovery to combat antimicrobial resistance. Trends in Biochemical Sciences, 44(11):961-972.
  • Novaković AR, Karaman MA, Milovanović IL, Belović MM, Rašeta MJ, Radusin TI, Ilić NM (2016) Edible mycorrhizal species Lactarius controversus Pers. 1800 as a source of antioxidant and cytotoxic agents. Hemijska Industrija, 70(2):113-122.
  • Özen T, Darcan C, Kaygusuz Ö, Türkekul İ (2016) The Chemical content, antioxidant and antimicrobial assays of Lactarius controversus and Lactarius musteus: two edible wild mushrooms from Giresun province of Turkey. Annals of Food Processing and Preservation, 1(1):1001.
  • Pancu DF, Scurtu A, Macasoi IG, Marti D, Mioc M, Soica C, Coricovac D, Horhat D, Poenaru M, Dehelean C (2021) Antibiotics: conventional therapy and natural compounds with antibacterial activity a pharmaco-toxicological screening. Antibiotics, 10(4):401.
  • Pekşen A, Kibar B, Yakupoğlu G (2007) Yenilebilir bazı Lactarius türlerinin morfolojik özelliklerinin, protein ve mineral içeriklerinin belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 22(3):301-305.
  • Stanković M, Mitić V, Stankov Jovanović V, Dimitrijević M, Nikolić J, Stojanović G (2022) Selected fungi of the genus Lactarius screening of antioxidant capacity, antimicrobial activity, and genotoxicity. Journal of Toxicology and Environmental Health, Part A, 85(17):699-714.
  • Suleiman WB, Shehata RM, Younis AM (2022) In vitro assessment of multipotential therapeutic importance of Hericium erinaceus mushroom extracts using different solvents. Bioresources and Bioprocessing, 9(1):1-13.
There are 20 citations in total.

Details

Primary Language Turkish
Subjects Botany (Other)
Journal Section Research Article
Authors

Şule İnci 0000-0002-4022-5269

Sevda Kırbağ 0000-0002-4337-8236

Publication Date May 15, 2024
Acceptance Date November 12, 2023
Published in Issue Year 2024Volume: 25 Issue: 1

Cite

APA İnci, Ş., & Kırbağ, S. (2024). Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 25(1), 1-5. https://doi.org/10.17474/artvinofd.1335292
AMA İnci Ş, Kırbağ S. Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi. ACUJFF. May 2024;25(1):1-5. doi:10.17474/artvinofd.1335292
Chicago İnci, Şule, and Sevda Kırbağ. “Lactarius Controversus (Pers.) Pers.’in Antimikrobiyal Etkisinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25, no. 1 (May 2024): 1-5. https://doi.org/10.17474/artvinofd.1335292.
EndNote İnci Ş, Kırbağ S (May 1, 2024) Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25 1 1–5.
IEEE Ş. İnci and S. Kırbağ, “Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi”, ACUJFF, vol. 25, no. 1, pp. 1–5, 2024, doi: 10.17474/artvinofd.1335292.
ISNAD İnci, Şule - Kırbağ, Sevda. “Lactarius Controversus (Pers.) Pers.’in Antimikrobiyal Etkisinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25/1 (May 2024), 1-5. https://doi.org/10.17474/artvinofd.1335292.
JAMA İnci Ş, Kırbağ S. Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi. ACUJFF. 2024;25:1–5.
MLA İnci, Şule and Sevda Kırbağ. “Lactarius Controversus (Pers.) Pers.’in Antimikrobiyal Etkisinin Belirlenmesi”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, vol. 25, no. 1, 2024, pp. 1-5, doi:10.17474/artvinofd.1335292.
Vancouver İnci Ş, Kırbağ S. Lactarius controversus (Pers.) Pers.’in antimikrobiyal etkisinin belirlenmesi. ACUJFF. 2024;25(1):1-5.
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