Research Article
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Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines

Year 2024, Volume: 25 Issue: 1, 104 - 110, 15.05.2024
https://doi.org/10.17474/artvinofd.1403978

Abstract

The genus Lallemantia (Lamiaceae) is popularly known as "Dragonhead". The genus, which has been proven to have extremely rich compounds, is used in the treatment of many diseases among the people. The genus is represented by 5 taxa in the world and 3 taxa are naturally distributed in Türkiye. In this study, the cytotoxic properties of methanolic extracts of the above-ground parts of Lallemantia peltata, Lallemantia iberica and Lallemantia canescens taxa were investigated against BEAS-2B, SH-SY5Y, HCT 116 and Hep G2 cell lines. Changes in the viability of cancer cells were determined using the MTT method. The results showed that all three species had varying degrees of cytotoxic effect. This effect was maximum in L. canescens with 53.54% (p<0.001) on HCT 116, 37.53% (p=0.005) on SH-SY5Y and 32.03% (p=0.026) on Hep G2 at 800 μg/ml and minimum in L. iberica. In addition, the herbal extracts had no toxic effect on healthy lung cells (BEAS-2B).

Ethical Statement

-

Supporting Institution

TUBITAK

Project Number

TUBITAK-1919B012100464

Thanks

This study was supported by TUBITAK as project number 1919B012100464.

References

  • Alan Y, Savcı A, Koçpınar EF, Kurşat M, Topdemir S, Karataş M, Çakmak B (2019) Antimicrobial, antioxidant and DNA protective effects and phenolic content of Lallementia canescens (L.) Fisch. & C.A.Mey. and Lallementia peltata (L.) Fisch. & C.A.Mey. Biological Diversity and Conservation, 12(3):78-88.
  • Altın S, Akyüz S, Yeniçeri EKK, Köksal E, Altay A, Alp C (2021) Investigation of Antimicrobial Activity of Lallemantia canescens (L.) Fisch & Mey. Erzincan University Journal of Science and Technology, 14(2): 849-856.
  • Badu-Gyan F, Owusu V (2017) Consumer willingness to pay a premium for a functional food in Ghana. Applied Studies in Agribusiness and Commerce, 11(1-2): 51-59.
  • Başak SŞ, Candan F (2008) Lallemantia canescens (L) Fisch & Mey bitkisinin ve kallus doku kültürünün antioksidan aktivitesi. İstanbul Teknik Üniversitesi Dergisi/C Fen Bilimleri, 6(1): 14-28.
  • Baykara O (2016) Kanser tedavisinde güncel yaklaşımlar. Balıkesir Sağlık Bilimleri Dergisi, 5(3):154-165.
  • Bray F, Møller B (2006) Predicting the future burden of cancer. Nature Reviews Cancer, 6(1): 63-74.
  • Çakiroğlu FP, Uçar A (2018) Consumer attitudes towards purchasing functional products. Progress in Nutrition, 20: 257-262.
  • Devasagayam TPA, Boloor KK, Ramsarma T (2003) Methods for estimating lipid peroxidation: analysis of merits and demerits (minireview). Indian Journal of Biochemistry and Biophysics, 40(5):300-308.
  • Freimoser FM, Jakob CA, Aebi M, Tuor U (1999) The MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities. Applied and Environmental Microbiology, 65(8): 3727-3729.
  • IBM Corp (2020) IBM SPSS statistics for windows (version 22.0) [computer software]. IBM Corp.
  • Jamzad Z, Grayer RJ, Kite GC (2003) Leaf Surface flavonoids in Iranian species of Nepata (Lamiaceae) and some related genera. Bıochemical Systematics Ecology, 31(6): 587- 600.
  • Kahraman A, Serteser M, Koken T (2002) Flavonoıds. The Medical Journal of Kocatepe, 3: 1-8.
  • Karami L, Ghahtan N, Habibi H (2017) Antibacterial effect of Plantago ovata and Lallemantia iberica seed extracts against some bacteria. Research in Molecular Medicine, 5(3): 32-36.
  • Kirbag S, Keser S, Tekin S, İnci Ş, Sandal S (2021) Cytotoxic effect of endemic Tchihatchewia isatidea Boiss. from Türkiye. Israel Journal of Plant Science, 68(1-2):161-165.
  • Rahimi A, Ahmadi F, Akbarpour L (2019) A Comparison of some phytochemical characteristics of five types of Lallemantia iberica seeds from different parts of Azerbaijan Region. 1. International Field Crops Congress, Antalya/Türkiye pp:122.
  • Riss TL, Moravec RA (2004) Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay And Drug Development Technologies, 2(1): 51-62.
  • Sarma AD, Mallick AR, Ghosh AK (2010) Free radicals and their role in different clinical conditions: an overview. International Journal of Pharmaceutical Sciences and Research, 1(3): 185-192.
  • Sefidkon F, Sonboli A, Kalvandi R (2006) Analysis of the essential oil of Lallemantia peltata from Iran. Journal of Essential Oil Bearing Plants, 9(1):42-46.
  • Semnani KM (2006) Essential oil composition of Lallemantia iberica Fisch. et C.A. Mey. Journal of Essential Oil Research, 18(2): 689-690.
  • Siegel RL, Miller KD, Wagle NS, Jemal A (2023) Cancer statistics. CA: A Cancer Journal for Clinicians, 73(1): 17-48.
  • Tekin S, Beytur A, Çakır M, Tekin Ç, Sandal S (2017) Saksagliptin ve sitagliptinin farklı tip insan kanser hücre canlılığı üzerine etkilerinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, 31(3): 111-116.
  • URL-1 https://www.drozdogan.com/dunya-kanser-istatistikleri-2020-her-5-kisiden-biri-kanserle-karsilasiyor/, Acessed: 11.10.2023.
  • URL-2 GLOBOCAN 2020 Database. https://gco.iarc.fr/, Accessed: 10.09.2023.
  • van Meerloo J, Kaspers GJ, Cloos J (2011) Cell sensitivity assays: the MTT assay. Methods Molecular Biology, 731: 237-245.
  • van Tonder A, Joubert AM, Cromarty AD (2015) Limitations of the 3-(4, 5- dimethylthiazol-2- yl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared tothree commonly used cell enumeration assays. BMC Research Notes, 8(1): 47-60.
  • Wamidh HT, Mahasneh AM (2010) Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia Pharmaceutica, 78: 33-45.
  • Yener I, Olmez OT, Ertaş A, Yılmaz Ma, Fırat M, Kandemir SI, Öztürk M (2018) A detailed study on chemical and biological profile of nine Euphorbia species from Türkiye with chemometric approach: Remarkable cytotoxicity of E. fistulasa and promising tannic acid content of E. eriophora. Industrial Crops and Products, 123: 442-453.

Türkiye'de yetişen Lallemantia Fish. & C.A. Mey. türlerinin çeşitli kanser hücre hatları üzerindeki sitotoksik etkisinin araştırılması

Year 2024, Volume: 25 Issue: 1, 104 - 110, 15.05.2024
https://doi.org/10.17474/artvinofd.1403978

Abstract

Lallemantia cinsi (Lamiaceae) halk arasında “Ajdarbaşı” olarak bilinir. Son derece zengin bileşiklere sahip olduğu kanıtlanmış olan cins, halk arasında birçok rahatsızlığın tedavisinde kullanılmaktadır. Dünyada 5 taksonla temsil edilen cinsin 3 taksonu Türkiye’de doğal yayılış göstermektedir. Bu çalışmada, Lallemantia peltata, Lallemantia iberica ve Lallemantia canescens taksonlarının toprak üstü kısımlarının metanolik ekstraktının BEAS-2B, SH-SY5Y, HCT 116 ve Hep G2 hücre hatlarına karşı sitotoksik özellikleri araştırılmıştır. Kanser hücrelerinin canlılığındaki değişiklikler ise, MTT yöntemi kullanılarak tespit edilmiştir. Sonuçlar, her üç türün de değişen oranlarda sitotoksik etkiye sahip olduğunu göstermiştir. Bu etki; 800 μg/ml'de HCT 116 üzerinde %53.54 (p<0.001), SH-SY5Y üzerinde %37.53 (p=0.005) ve Hep G2 üzerinde %32.03 (p=0.026) ile bu etkinin L. canescens'te maksimum ve L. iberica'da minimum olduğu gözlendi. Ayrıca bitkisel ekstraktların sağlıklı akciğer hücreleri (BEAS-2B) üzerinde ise herhangi bir toksik etki yaratmadığı gözlemlendi.

Ethical Statement

-

Supporting Institution

TUBITAK

Project Number

TUBITAK-1919B012100464

Thanks

Bu çalışma TUBITAK tarafından 1919B012100464 numaralı proje olarak desteklenmiştir.

References

  • Alan Y, Savcı A, Koçpınar EF, Kurşat M, Topdemir S, Karataş M, Çakmak B (2019) Antimicrobial, antioxidant and DNA protective effects and phenolic content of Lallementia canescens (L.) Fisch. & C.A.Mey. and Lallementia peltata (L.) Fisch. & C.A.Mey. Biological Diversity and Conservation, 12(3):78-88.
  • Altın S, Akyüz S, Yeniçeri EKK, Köksal E, Altay A, Alp C (2021) Investigation of Antimicrobial Activity of Lallemantia canescens (L.) Fisch & Mey. Erzincan University Journal of Science and Technology, 14(2): 849-856.
  • Badu-Gyan F, Owusu V (2017) Consumer willingness to pay a premium for a functional food in Ghana. Applied Studies in Agribusiness and Commerce, 11(1-2): 51-59.
  • Başak SŞ, Candan F (2008) Lallemantia canescens (L) Fisch & Mey bitkisinin ve kallus doku kültürünün antioksidan aktivitesi. İstanbul Teknik Üniversitesi Dergisi/C Fen Bilimleri, 6(1): 14-28.
  • Baykara O (2016) Kanser tedavisinde güncel yaklaşımlar. Balıkesir Sağlık Bilimleri Dergisi, 5(3):154-165.
  • Bray F, Møller B (2006) Predicting the future burden of cancer. Nature Reviews Cancer, 6(1): 63-74.
  • Çakiroğlu FP, Uçar A (2018) Consumer attitudes towards purchasing functional products. Progress in Nutrition, 20: 257-262.
  • Devasagayam TPA, Boloor KK, Ramsarma T (2003) Methods for estimating lipid peroxidation: analysis of merits and demerits (minireview). Indian Journal of Biochemistry and Biophysics, 40(5):300-308.
  • Freimoser FM, Jakob CA, Aebi M, Tuor U (1999) The MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities. Applied and Environmental Microbiology, 65(8): 3727-3729.
  • IBM Corp (2020) IBM SPSS statistics for windows (version 22.0) [computer software]. IBM Corp.
  • Jamzad Z, Grayer RJ, Kite GC (2003) Leaf Surface flavonoids in Iranian species of Nepata (Lamiaceae) and some related genera. Bıochemical Systematics Ecology, 31(6): 587- 600.
  • Kahraman A, Serteser M, Koken T (2002) Flavonoıds. The Medical Journal of Kocatepe, 3: 1-8.
  • Karami L, Ghahtan N, Habibi H (2017) Antibacterial effect of Plantago ovata and Lallemantia iberica seed extracts against some bacteria. Research in Molecular Medicine, 5(3): 32-36.
  • Kirbag S, Keser S, Tekin S, İnci Ş, Sandal S (2021) Cytotoxic effect of endemic Tchihatchewia isatidea Boiss. from Türkiye. Israel Journal of Plant Science, 68(1-2):161-165.
  • Rahimi A, Ahmadi F, Akbarpour L (2019) A Comparison of some phytochemical characteristics of five types of Lallemantia iberica seeds from different parts of Azerbaijan Region. 1. International Field Crops Congress, Antalya/Türkiye pp:122.
  • Riss TL, Moravec RA (2004) Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay And Drug Development Technologies, 2(1): 51-62.
  • Sarma AD, Mallick AR, Ghosh AK (2010) Free radicals and their role in different clinical conditions: an overview. International Journal of Pharmaceutical Sciences and Research, 1(3): 185-192.
  • Sefidkon F, Sonboli A, Kalvandi R (2006) Analysis of the essential oil of Lallemantia peltata from Iran. Journal of Essential Oil Bearing Plants, 9(1):42-46.
  • Semnani KM (2006) Essential oil composition of Lallemantia iberica Fisch. et C.A. Mey. Journal of Essential Oil Research, 18(2): 689-690.
  • Siegel RL, Miller KD, Wagle NS, Jemal A (2023) Cancer statistics. CA: A Cancer Journal for Clinicians, 73(1): 17-48.
  • Tekin S, Beytur A, Çakır M, Tekin Ç, Sandal S (2017) Saksagliptin ve sitagliptinin farklı tip insan kanser hücre canlılığı üzerine etkilerinin araştırılması. Fırat Üniversitesi Sağlık Bilimleri Tıp Dergisi, 31(3): 111-116.
  • URL-1 https://www.drozdogan.com/dunya-kanser-istatistikleri-2020-her-5-kisiden-biri-kanserle-karsilasiyor/, Acessed: 11.10.2023.
  • URL-2 GLOBOCAN 2020 Database. https://gco.iarc.fr/, Accessed: 10.09.2023.
  • van Meerloo J, Kaspers GJ, Cloos J (2011) Cell sensitivity assays: the MTT assay. Methods Molecular Biology, 731: 237-245.
  • van Tonder A, Joubert AM, Cromarty AD (2015) Limitations of the 3-(4, 5- dimethylthiazol-2- yl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared tothree commonly used cell enumeration assays. BMC Research Notes, 8(1): 47-60.
  • Wamidh HT, Mahasneh AM (2010) Antiproliferative activity of plant extracts used against cancer in traditional medicine. Scientia Pharmaceutica, 78: 33-45.
  • Yener I, Olmez OT, Ertaş A, Yılmaz Ma, Fırat M, Kandemir SI, Öztürk M (2018) A detailed study on chemical and biological profile of nine Euphorbia species from Türkiye with chemometric approach: Remarkable cytotoxicity of E. fistulasa and promising tannic acid content of E. eriophora. Industrial Crops and Products, 123: 442-453.
There are 27 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Research Article
Authors

Ali Kaya Çelik 0009-0004-6895-9995

Pelin Yılmaz Sancar 0000-0002-6134-622X

Irmak Icen Taskın 0000-0002-1612-0563

Murat Kürşat 0000-0002-0861-4213

Project Number TUBITAK-1919B012100464
Publication Date May 15, 2024
Submission Date December 12, 2023
Acceptance Date April 3, 2024
Published in Issue Year 2024Volume: 25 Issue: 1

Cite

APA Çelik, A. K., Yılmaz Sancar, P., Icen Taskın, I., Kürşat, M. (2024). Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 25(1), 104-110. https://doi.org/10.17474/artvinofd.1403978
AMA Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M. Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. ACUJFF. May 2024;25(1):104-110. doi:10.17474/artvinofd.1403978
Chicago Çelik, Ali Kaya, Pelin Yılmaz Sancar, Irmak Icen Taskın, and Murat Kürşat. “Investigation of the Cytotoxic Effect of Lallemantia Fisch. & C.A. Mey. Species Growing in Türkiye on Various Cancer Cell Lines”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25, no. 1 (May 2024): 104-10. https://doi.org/10.17474/artvinofd.1403978.
EndNote Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M (May 1, 2024) Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25 1 104–110.
IEEE A. K. Çelik, P. Yılmaz Sancar, I. Icen Taskın, and M. Kürşat, “Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines”, ACUJFF, vol. 25, no. 1, pp. 104–110, 2024, doi: 10.17474/artvinofd.1403978.
ISNAD Çelik, Ali Kaya et al. “Investigation of the Cytotoxic Effect of Lallemantia Fisch. & C.A. Mey. Species Growing in Türkiye on Various Cancer Cell Lines”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi 25/1 (May 2024), 104-110. https://doi.org/10.17474/artvinofd.1403978.
JAMA Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M. Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. ACUJFF. 2024;25:104–110.
MLA Çelik, Ali Kaya et al. “Investigation of the Cytotoxic Effect of Lallemantia Fisch. & C.A. Mey. Species Growing in Türkiye on Various Cancer Cell Lines”. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, vol. 25, no. 1, 2024, pp. 104-10, doi:10.17474/artvinofd.1403978.
Vancouver Çelik AK, Yılmaz Sancar P, Icen Taskın I, Kürşat M. Investigation of the cytotoxic effect of Lallemantia Fisch. & C.A. Mey. species growing in Türkiye on various cancer cell lines. ACUJFF. 2024;25(1):104-10.
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